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相关概念视频

Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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Protein Folding01:22

Protein Folding

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Overview
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Protein Organization01:13

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Assembly of Cytoskeletal Filaments01:18

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Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
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相关实验视频

Updated: Jul 4, 2025

A Protocol for Computer-Based Protein Structure and Function Prediction
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A Protocol for Computer-Based Protein Structure and Function Prediction

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CombFold:使用组合组合算法和AlphaFold2预测大型蛋白质组合的结构.

Ben Shor1, Dina Schneidman-Duhovny2

  • 1The Rachel and Selim Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel.

Nature methods
|February 7, 2024
PubMed
概括
此摘要是机器生成的。

使用AlphaFold2子单元相互作用,CombFold可以准确地预测大型蛋白质复杂结构. 这种新方法改善了结构覆盖,并支持实验数据集成,用于复杂的组装预测.

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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

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相关实验视频

Last Updated: Jul 4, 2025

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16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

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科学领域:

  • 结构生物学是结构生物学.
  • 计算生物学是一种计算生物学.
  • 生物物理学的生物物理.

背景情况:

  • 像AlphaFold2和RosettaFold这样的深度学习模型在单质蛋白质结构预测方面表现出色.
  • 由于其规模和子单元相互作用的复杂性,预测大型蛋白质复合物的结构仍然是一个重大挑战.

研究的目的:

  • 开发一种新的算法,CombFold,用于准确预测大型蛋白质复杂结构.
  • 为了提高蛋白质复合体的结构覆盖范围,超出单个子单元的预测.

主要方法:

  • CombFold采用一种组合和层次的组装策略.
  • 它利用了AlphaFold2.2预测的双对子单元相互作用.
  • 该算法将距离限制与交联质谱相结合,并列出石化测量.

主要成果:

  • 在前10个预测中,CombFold在72%的大型不对称组件中实现了高精度 (TM分数>0.7).
  • 预测的复杂结构显示结构覆盖率比蛋白质数据库条目大20%.
  • 对于具有已知静态度但未知结构的复合体,获得了高可信度预测.

结论:

  • CombFold是一个强大的工具,用于预测大型蛋白质复合物的结构.
  • 该方法显著扩大了结构覆盖范围,超出了单蛋白.
  • CombFold 便于实验数据的整合,以实现更准确的复杂建模.