染料的pH取决和全细胞催化脱色,使用来自Rhodococcus jostii的重组染料脱色过氧化酶
Xiaoyan Duan1, Qian Pi1, Lei Tang2,3
1Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, No 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China.
Bioprocess and biosystems engineering
|February 7, 2024
概括
罗多科克 (Rhodococcus jostii) 染料脱色过氧化酶 (RhDyPB) 有效地降解了像马拉绿这样的染料. 在大肠杆菌中过度表达RhDyPB可增强废水中的染料生物修复.
科学领域:
- 环境微生物学 环境微生物学
- 生物催化剂是一种生物催化剂.
- 废水处理 废水处理
背景情况:
- 废水中的染料对环境和健康构成风险.
- 染料脱色过氧化酶 (DyP) 显示出染料降解的潜力,但影响其效率的因素尚不清楚.
研究的目的:
- 为了研究 Rhodococcus jostii 染料脱色过氧化酶 (RhDyPB) 的脱色能力.
- 探索RhDyPB介导的染料降解的机制和影响因素.
- 评估整个大肠杆菌细胞过度表达RhDyPB用于染料生物修复的应用.
主要方法:
- 在大肠杆菌中过度表达RhDyPB.
- 使用四种不同的染料,用纯化的RhDyPB和整个大肠杆菌细胞进行脱色试验.
- 基于pH值的活性分析.
- 电子喷射电离质谱仪 (ESI-MS) 用于中间分析.
- 分子对接用于机制预测.
主要成果:
- 纯化RhDyPB表现出pH取决于染料色色的变色,马六合绿在pH 6.0时具有最佳活性 (94.4%变色).
- ESI-MS证实了反应蓝色19降解中的过氧化酶催化.
- 分子对接表明了基因转移机制.
- 与对照细胞相比,过度表达RhDyPB的整个大肠杆菌细胞显示了反应蓝色19,色卡尔明和马六合绿色的降解率有所改善.
结论:
- RhDyPB 是一种有效的生物催化剂,用于降解各种染料.
- 在大肠杆菌中RhDyPB的表达增强了其对含有染料的废水生物修复的潜力.
- 全细胞系统为实际的染料脱色应用提供了可行的方法.
关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.罗多科库斯 (Rhodococcus jostii) 是一个有趣的植物.含有染料的废水.分子对接是分子对接.整个细胞的催化作用.更多相关视频
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