胚胎-子宫内膜相互作用与胚胎在马的移动阶段的位置相关
Thadeu de Castro1, Machteld van Heule1,2, Rafael R Domingues3
1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Scientific reports
|February 7, 2024
概括
移动的马类胚胎会影响局部子宫内膜基因表达. 这种相互作用对于确定怀孕至关重要,影响与生长和信号传递相关的途径.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 动物科学动物科学
背景情况:
- 胚胎与母亲的交叉通话对于成功建立怀孕至关重要.
- 在马匹中,胚胎在排卵后9-15天之间在子宫内迁移.
- 假设这种迁移会诱导局部子宫内膜变化.
研究的目的:
- 为了研究移动胚胎对马体内膜基因表达的影响.
- 为了确定受胚胎存在影响的特定基因和途径.
主要方法:
- 对子宫内膜组织进行比较性转录基因分析.
- 在第12天分析了三组:带有胚胎的孕角 (P+),没有胚胎的孕角 (P-),和非繁殖的马匹 (NB).
- 进行了差异基因表达分析.
主要成果:
- 在孕妇和非繁殖群体之间确定了超过1100个差异表达基因 (DEG).
- 孕期角 (vs. NB) 中的上调基因与生长因子和脂肪酸代谢有关.
- 与胚胎和没有胚胎的孕妇角进行比较,发现了59个DEG,其中30个基因在胚胎存在时升调,与血管生长和免疫调节有关.
结论:
- 移动马类胚胎的存在局部改变子宫内膜基因表达.
- 这些变化涉及对怀孕早期至关重要的途径,包括血管化和免疫调节.
- 这项研究突出了胚胎与子宫内膜之间的动态相互作用.
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