在马拉被S. oneidensis MR-1生物降解期间氧化反应的机制
Qiaodong Pan1, Yanhong Li1,2,3, Jing Zhang1
1College of Environmental Science and Engineering, Guilin University of Technology, Jiangan Road 12, Guilin, 541004, Guangxi, China.
Environmental science and pollution research international
|February 7, 2024
概括
谢瓦尼拉oneidensisMR-1有效降解有毒农药马拉. 马拉会引起氧化应激并改变细胞特性,但细菌表现出耐药性和生物修复潜力.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物化学 生化学
背景情况:
- 马拉是一种广泛使用的有机农药,对人类和环境有着显著的毒性.
- 谢瓦尼拉 (Shewanella oneidensis) MR-1因其对各种污染物的生物修复能力而闻名.
- 在S. oneidensis MR-1对马拉的生理和生化反应仍然在很大程度上没有特征.
研究的目的:
- 为了研究Shewanella oneidensis MR-1对马拉暴露的生理和生化反应.
- 为了评估S. oneidensis MR-1在马拉上的生物降解效率.
- 了解马拉降解背后的机制以及S. oneidensis MR-1中相关的细胞变化.
主要方法:
- 评估7天内不同度 (10,20,30毫克/升) 的马拉降解率.
- 测量酶活性 (超氧化物脱酶,酶) 和含量,以评估氧化应激.
- 分析反应性氧物种 (ROS) 生产,可溶性多糖分泌,细胞密度,细胞内蛋白质和ATPase水平 (Na+/K+-ATPase,Ca2+/Mg2+-ATPase).
- 使用扫描电子显微镜 (SEM) 来观察细胞形态和生物质的变化.
主要成果:
- 在7天内,S. oneidensis MR-1 实现了高马拉降解率 (84.1%至94.0%).
- 马拉暴露抑制了超氧化物脱酶和酶,增加了甲基,并增加了ROS产量,表明氧化应激.
- ROS刺激可溶性多糖分泌,有助于减轻氧化应激.
- 初始暴露于马拉会增加细胞密度和蛋白质含量,表现出耐药性,但长期暴露导致生物质减少,细胞活性和破裂.
- 马拉诱导的氧化应激干扰了能量代谢,由降低的Na+/K+-ATPase和Ca2+/Mg2+-ATPase水平证明.
结论:
- 谢瓦尼拉oneidensisMR-1显示了马拉生物修复的显著潜力.
- 马拉在S. oneidensis MR-1中诱导氧化应激,影响酶活动,ROS水平和细胞组件.
- 该细菌采用诸如多糖分泌等防御机制,并表现出耐药性,但高度会导致细胞损伤和能量代谢受损.
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