提高肠道病原体检测:多重PCR的实施和影响,以改善诊断和监测
Jad Mohtar1, Hiba Mallah1, Jean Marc Mardirossian1,2
1Faculty of Health Sciences, University of Balamand, Beirut, Lebanon.
BMC infectious diseases
|February 7, 2024
概括
在黎巴嫩,对急性胃肠炎的综合征监测显示了高的病原体检测率,其中大肠杆菌等细菌最常见. 这凸显了对肠道感染的全面监测的必要性.
科学领域:
- 医学微生物学 医学微生物学
- 传染性疾病 传染性疾病
- 公共卫生监督 公共卫生监督
背景情况:
- 急性胃肠炎是全球死亡的主要原因,特别是在发展中国家.
- 黎巴嫩缺乏急性胃肠炎的国家监测,对引起腹的病原体的数据有限.
- 这项研究解决了对黎巴嫩胃肠道感染的流行病学数据的需求.
研究的目的:
- 在黎巴嫩调查常见胃肠道病原体的流行病学.
- 将发现与有关导致腹的物质的现有数据进行比较.
- 为未来的公共卫生干预建立基线数据.
主要方法:
- 一个多中心的,跨截面研究进行了一年.
- 从急性胃肠炎症状的门诊患者和住院患者收集了271个样本.
- 使用Allplex胃肠道测定用于检测肠道病原体的分析.
主要成果:
- 在71%的病例中检测到肠病原体,54%是混合感染.
- 细菌 (48%) 是最常见的,其次是寄生虫 (12%) 和病毒 (11%).
- 最常见的病原体:大肠杆菌菌株 (EAEC,ETEC,EPEC),人体细胞囊和罗塔病毒. 与罗塔病毒,ETEC,BH和EPEC相关的高住院率.
结论:
- 多重实时PCR测定提高了病原体检测率,并发现了监测差距.
- 综合监测对于监测肠道感染至关重要.
- 综合征测试小组为有效的治疗和公共卫生策略提供及时的数据.
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