相关实验视频

Updated: Jul 4, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

动态N6 - - 甲基氨酸RNA修饰调节花生对细菌枯的抵抗力

Kai Zhao¹, Zhongfeng Li¹, Yunzhuo Ke²

¹College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou, 450046, China.

The New phytologist

|February 8, 2024

概括

在花生植物中,N6-甲基氨酸 (m6A) 修饰调节了细菌枯感染期间的基因表达. 一种特定的脱甲基酶,AhALKBH15,可提高抗性基因的调节,增强对Ralstonia solanacearum的防御能力.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
植物病原体相互作用

背景情况:

N6-甲基氨酸 (m6A) 是一种流行的mRNA修饰,可以调节真核生物中的基因表达.
在花生 (Arachis hypogea L.) 抵抗Ralstonia solanacearum引起的细菌枯 (BW) 的作用中m6A的作用尚不清楚.

研究的目的:

为了研究m6A在花生对R. solanacearum感染的反应中的动态和功能.
在花生中识别m6A基因和相关基因.

主要方法:

在R. solanacearum感染后,对抗性和易受性花生附加的m6A水平的比较分析.
RNA测序和m6A甲基组分析.
鉴定m6A基因和对m6A相关基因的功能丰富分析.

主要成果:

在花生中发现了保存的m6A图案"URUAY".
不同的m6A修饰主要在3' UTR和外显子中发现.
与m6A相关的基因在植物病原体相互作用途径中得到显著丰富.
确定AhALKBH15是一种m6A脱甲基酶,降低m6A水平并上调抗性基因AhCQ2G6Y.

结论:

m6A修饰在花生对抗细菌的防御中发挥作用.
通过AhALKBH15介导的脱甲基化和随后的AhCQ2G6Y上调有助于提高花生中的BW耐药性.

关键词:

由于细菌的原因,它们会枯.脱甲基酶是什么脱甲基酶疾病耐药性疾病耐药性 m6A 修改的修改花生花生花生花生花生

更多相关视频

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

Pre-mRNA Processing: Modification of pre-mRNA Ends

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...