相关实验视频

Updated: Jul 4, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

豆类常见马赛克病毒分离物的分子表征,该病毒首次在花园中识别,使用元转录组和小RNA测序

Zhong-Tian Xu¹, Hai-Tao Weng¹, Jian-Ping Chen¹

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

The plant pathology journal

|February 8, 2024

概括

豆类常见马赛克病毒 (BCMV) 首次在花园植物中新发现. 这一发现为BCMV提供了关于BCMV的见解.

科学领域:

植物病毒学植物病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

花 (Gardenia jasminoides) 是一种重要的装饰植物和药用植物.
之前没有关于植物病毒存在于加德尼亚的记录.
在种植的花园植物中观察到病毒性疾病症状.

研究的目的:

为了识别和描述影响花园植物的病毒.
测序和分析任何已识别的病毒的基因组.
了解对BCMV多样性和宿主范围的影响.

主要方法:

感染加德尼叶的转基因组测序.感染加德尼叶的转基因组测序.
感染的花园花叶的小RNA测序.
对测序数据的生物信息分析,包括遗传学分析.

主要成果:

豆类常见马赛克病毒 (BCMV) 首次在加德尼亚被发现,被命名为BCMV-gardenia.
测序了BCMV-gardenia (10,054 nt) 的完整基因组,编码了3,222个氨基酸聚蛋白.
遗传学分析显示了BCMV-gardenia集群与非豆植物的BCMV分离物.
病毒衍生的小干扰RNAs (vsiRNAs) 主要为21-22nt,在5'终端偏好U/A.

结论:

这是BCMV感染Gardenia的第一个报告.
与其他分离物相比,BCMV-gardenia在其N末端显示出明显的序列特征.
这些发现表明BCMV的潜在宿主扩张.
这项研究为新主机中BCMV管理提供了基础数据.

关键词:

豆豆常见的马赛克病毒加德尼亚花园的花园.转录元组 (metatranscriptome) 是一个转录元组.病毒衍生的小干扰RNA.

更多相关视频

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...