在玉米线粒体和塑体中编辑PPR-E+向部位时,多种因素相互作用
Yong Wang1, Zi-Qin Huang1, Kai-Di Tian1
1Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China.
Plant communications
|February 8, 2024
概括
玉米RNA编辑因子ZmNUWA和ZmDYW2A/2B对于种子发育和器官基因表达至关重要. 它们相互作用,形成线粒体和塑体编码体的基本组成部分.
科学领域:
- 植物分子生物学 植物分子生物学
- 有机细胞的基因表达.
- 编辑RNA机制的RNA编辑机制
背景情况:
- 植物器官中的丁-丁编辑对于基因表达至关重要.
- 据认为,五基重复 (PPR) -E+蛋白向RNA位点,并招募像AtDYW2这样的cytidine deaminases,而AtNUWA则促进了这一过程.
研究的目的:
- 为了研究玉米NUWA (ZmNUWA) 和DYW2 (ZmDYW2A,ZmDYW2B) 同类的功能.
- 阐明它们在玉米细胞器RNA编辑中的作用和与其他编辑因子的相互作用.
主要方法:
- 在玉米中对ZmDYW2A,ZmDYW2B和ZmNUWA进行突变分析.
- 对ZmNUWA,ZmDYW2A和ZmDYW2B的亚细胞局部化研究.
- 蛋白质与蛋白质相互作用测试 (共免疫沉,酵母双杂交).
主要成果:
- ZmDYW2A/2B和ZmNUWA对玉米种子发育至关重要;单个突变是可行的,但双 / 三重突变显示严重的发育停止.
- ZmNUWA,ZmDYW2A和ZmDYW2B在线粒体和塑体中具有双重局部.
- 丧失ZmNUWA会影响许多线粒体和塑位的编辑,而丧失ZmDYW2A/2B会影响PPR-E+蛋白准的位点.
- ZmNUWA与ZmDYW2A/2B互动,暗示它招募他们进行编辑.
- ZmNUWA和ZmDYW2A/2B与MORF蛋白 (ZmMORF1,ZmMORF2,ZmMORF8,ZmMORF9) 相互作用,而ZmOZ1与ZmORRM1和其他因素相互作用,定义线粒体和塑结核体的组成部分.
结论:
- ZmNUWA和ZmDYW2A/2B对于玉米器官RNA编辑和植物发育是不可或缺的.
- 这些蛋白质与MORF和其他因素一起,在线粒体和塑体中形成明显的编辑体复合体.
- 这些发现为玉米器官中的PPR-E+向RNA编辑机器提供了一个全面的模型.
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