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在复杂矩阵中无标签检测甲基转移酶活动的化RNA的基于aptamer的放大和转录策略

Yu Gu¹, Cunxia Fan¹, Hongbin Yang¹

¹Institute for Materials Science and Devices, School of Materials Science and Engineering, Suzhou University of Science and Technology, Kerui Road, Suzhou, 215009, P.R. China.

Advanced biology

|February 8, 2024

概括

一种新型的光RNA吸收体传感器可以无标签检测DNA甲基转移酶 (M.SssI). 这种敏感的方法为癌症诊断和治疗提供了一个有前途的工具,通过在生物样本中准确量化M.SssI.

科学领域:

生物化学生物化学
分子生物学分子生物学
癌症研究癌症研究

背景情况:

基因甲基转移酶 (DNMT) 在基因表达和细胞功能中起着至关重要的作用.
异常DNMT表达与癌症发病和进展有关.
在癌症诊断和治疗中需要敏感的,无标签的DNMT评估方法.

研究的目的:

开发一种新的,无标签的光传感器,用于对DNA甲基转移酶 (M.SssI) 的敏感量化.
建立用于癌症研究的复杂生物矩阵中M.SssI确定的一种实用工具.

主要方法:

采用了一种基于RNA胺体的化照明感应策略.
该方法利用限制性内核酶,基于挂锁的识别和RNA转录.
滚动圆放大 (RCA) 和RNA转录与菜染料RNA吸收体相结合,用于光反应.

主要成果:

开发的平台表现出对M.SssI.的超高灵敏度和选择性.
实现了0.02100 U mL-1的线性检测范围,低检测极限为1.6 × 10−3 U mL-1.
这种方法在应用于的血清样本时,显示出高准确性 (92.0107.0%恢复) 和精度 (<7.0%RSD).

结论:

基于RNA体的光传感器提供了一种高度敏感和选择性的方法,用于无标签的M.SssI量化.
这种方法为复杂的生物样本中M.SssI的确定提供了有希望和实用的工具,有助于癌症诊断和治疗.
开发的传感平台满足了对瘤诊断和癌症治疗监测中可靠技术的需求.

关键词:

化RNA的阿帕特默是化RNA的阿帕特默甲基转移酶的使用方法基于挂锁的识别系统限制内核酶的限制菜,西班牙菜.

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