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Updated: Jul 4, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

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基于AuNPs固定DNA探针的近距离结合,精确量化微RNAs

Keyu Li1, Peng Xiao1, Ningning Yuan1

  • 1Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zuoguowei@cqmu.edu.cn.

Analytical methods : advancing methods and applications
|February 8, 2024
PubMed
概括

我们开发了一种简单,灵敏的方法来检测microRNAs (miRNAs) 使用近距离结合和金纳米粒子. 这种测试可以准确地识别特定的miRNA,甚至可以区分单基变异,其检测极限为20aM.

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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

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相关概念视频

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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