低氧压力对番茄果细胞代谢反应的影响
Md Sultan Mahomud1, Md Nahidul Islam2,3, Joysree Roy1
1Department of Food Engineering and Technology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh.
Heliyon
|February 8, 2024
概括
番茄细胞中的低氧压力会改变新陈代谢,促进氨基酸和发酵,同时减少有机酸以求生存. 这会影响水果和蔬菜的收获后质量.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 收获后生物学 收获后生物学
背景情况:
- 收获后的水果和蔬菜损失通常是由细胞分解和色引起的.
- 控制大气储存可以诱导低氧 (低氧/无氧) 压力,导致这些损失.
- 了解细胞对氧气压力的反应对于改善储存至关重要.
研究的目的:
- 为了研究底层的新陈代谢机制孤立的西红果细胞对低氧压力的反应.
- 确定受低氧和无氧条件影响的关键代谢物.
- 为了将代谢物变化与氧气水平和化时间相关联.
主要方法:
- 隔离的番茄果细胞在受控的低氧 (低氧和无氧) 条件下进行化.
- 气色谱-质谱法 (GC-MS) 用于识别和量化极性代谢物.
- 采用多变量统计分析来确定关键代谢物和相关性.
主要成果:
- 氧气压力显著改变了56种已识别的极性代谢物的水平,包括氨基酸,有机酸和糖.
- 刺激了自由氨基酸的生物合成,同时减少了三碳酸循环中间体和大多数糖的合成.
- 在低氧条件下观察到糖酒精水平的增加和发酵代谢的诱导.
结论:
- 低氧压力会在番茄细胞中引发显著的代谢转变,通过改变能量代谢优先考虑生存.
- 特定的代谢物被确定为氧气应激反应的关键指标.
- 代谢物丰富度与氧气水平密切相关,为收获后储存提供了洞察力.
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