在宫样本中比较四种不同的人类乳头瘤病毒基因型定型方法:解决方法特定的优点和局限性
Juliana D Siqueira1, Brunna M Alves1, Adriana B C Castelo Branco1
1Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brazil.
Heliyon
|February 8, 2024
概括
通过比较HPV检测方法,实时PCR和桑格测序,发现人类乳头瘤病毒 (HPV) 和高风险HPV类型的鉴定具有很高的一致性. 下一代测序表明协议较低,影响宫癌查和预防策略.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 分子诊断学 分子诊断
背景情况:
- 人类乳头瘤病毒 (HPV) 是导致子宫癌和其他异位和口腔癌的主要原因.
- 鉴别流传的HPV类型对于全球有效的癌症查和预防计划至关重要.
研究的目的:
- 为了比较四种不同的HPV检测和基因定型方法的性能.
- 用实时PCR作为黄金标准,评估不同HPV检测方法的一致率.
主要方法:
- 实时PCR (Cobas®HPV测试) 测试可以进行.
- 嵌套PCR使用桑格测序.
- 反向杂交 (High®+Low PapillomaStrip®套件) 是一个非常简单的方法.
- 在Illumina HiSeq2500平台上使用下一代测序 (NGS).
主要成果:
- 与其他方法相比,下一代测序 (NGS) 对HPV和高风险HPV鉴定表现出最低的一致率 (67-75%).
- 实时PCR和桑格测序显示出最高的一致率 (97-100%).
- 所有评估的方法都显示了HPV检测灵敏度和特异性的变化.
结论:
- HPV 基因型鉴定对于个体风险评估,指导治疗干预,并为流行病学研究提供信息至关重要.
- 准确的HPV检测和基因定型方法对于制定有针对性的预防策略和疫苗开发至关重要.
- 方法选择显著影响HPV检测准确度,影响HPV感染管理中的公共卫生结果.
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