内皮-介质细胞转换,炎症和自的相互作用在多发性糖尿病视网膜病变的发病过程中
Gaocheng Zou1, Lijuan Que2, Yaping Liu3
1Department of Ophthalmology, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China.
Heliyon
|February 8, 2024
概括
内皮介质转变 (EndoMT) 与多发性糖尿病视网膜病变 (PDR) 有关. 这个过程涉及到原和VE-cadherin的变化,由炎症和自性缺陷调节,可能导致PDR.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 在患有多发性糖尿病视网膜病变 (PDR) 患者的视网膜内皮中研究了内皮-介质细胞过渡 (EndoMT).
- 该研究评估了视网膜和玻璃体样本中的EndoMT,并探索了其调节机制.
研究的目的:
- 评估EndoMT在PDR病变发生中的存在和意义.
- 确定关键的分子标记物和参与PDR相关EndoMT的调节途径.
主要方法:
- 从PDR和对照样本中对视网膜细胞 (CD31+) 的转录组测序,以确定差异表达基因 (DEGs).
- 从PDR患者和使用酶相关免疫吸收试验 (ELISA) 控制的玻璃体 (VH) 中选择EndoMT标记物的蛋白质表达分析.
主要成果:
- 确定了5845个DEG;分析集中在24个与EndoMT相关的基因上.
- 在PDR患者的VH中观察到中细胞标记物 (COL1A2) 的增加和内皮细胞标记物 (CDH5) 的减少.
- 在PDR患者中发现TGFβ,IL-1β,LC3B和P62的水平升高,这表明与炎症和自有关.
结论:
- 在PDR.的发病过程中,EndoMT与PDR.的发病有关.
- 在PDR中EndoMT的诱导和调节与自功能障碍和炎症反应有关.
- 抗VEGF治疗可能会影响与EndoMT相关的表型.
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