Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Fluid Mosaic Model01:19

Fluid Mosaic Model

11.7K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
11.7K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Membrane Fluidity01:26

Membrane Fluidity

11.2K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

APOE interacts with COX-2 on lipid droplets to modulate inflammatory lipid signaling.

bioRxiv : the preprint server for biology·2026
Same author

Endogenous neoantigen-specific antibodies mediate antitumor responses and determine vaccine efficacy.

Journal for immunotherapy of cancer·2026
Same author

Protein lipidation in cardiovascular homeostasis, development, and disease.

Clinical science (London, England : 1979)·2026
Same author

Tubulin polyglutamylation modulates Golgi morphodynamics and neurite branching during neuronal morphogenesis.

bioRxiv : the preprint server for biology·2026
Same author

Imaging interorganelle membrane contact sites using dimerization-dependent fluorescent proteins.

Methods in enzymology·2026
Same author

Tubulin acetylation governs organelle remodeling and lysosomal reformation during neuronal differentiation.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Jul 4, 2025

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
14:09

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

12.5K

联系FP:一个依赖于二元化的光蛋白工具包,用于可视化膜接触点动态.

Gregory E Miner1, Sidney Y Smith1, Wendy K Showalter1

  • 1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Contact (Thousand Oaks (Ventura County, Calif.))
|February 8, 2024
PubMed
概括

研究人员开发了Contact-FP,这是一种使用二分化依赖的光蛋白的新型工具,用于可视化活细胞中细胞器之间的膜接触点动态. 这项技术允许精确观察器官相互作用和细胞反应.

关键词:
生物传感器生物传感器洞穴 (caveolae) 是一个洞穴.细胞内膜网膜的内oplasmic网膜.光蛋白质是一种光蛋白质.脂质滴滴滴滴滴滴滴滴滴滴滴滴滴lysosomes 溶解体 溶解体 是一种溶解体.膜接触点与膜的接触点.线粒体中的线粒体.有机器人 有机器人过氧化物体是多氧化物体.血膜是一种等离子体膜.

更多相关视频

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

2.4K
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.3K

相关实验视频

Last Updated: Jul 4, 2025

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
14:09

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

12.5K
Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

2.4K
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.3K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 膜接触部位 (MCSs) 对于细胞通信至关重要,具有动态性,但难以研究.
  • 现有的可视化活细胞MCS动态的工具是有限的.
  • 分化依赖光蛋白 (ddFPs) 为MCS可视化提供了一个有前途的方法.

研究的目的:

  • 设计和验证一个名为Contact-FP的新型ddFP套件,用于可视化MCS动态.
  • 证明Contact-FP探针在研究器官相互作用,特别是脂质滴和线粒体之间的有用性.
  • 评估Contact-FP可视化复杂的MCS配置和干扰的能力.

主要方法:

  • 接触FP探针的工程针对各种有机体 (LDs,ER,线粒体,过氧体,溶酶体,等离子膜,洞穴,细胞质).
  • 定位研究以确认探头针对特定的有机体.
  • 活细胞成像可视化MCS形态和动态使用接触FP对.
  • 乱研究涉及过度表达器官连接器的研究 (例如,PLIN5).

主要成果:

  • 接触FP探头成功设计并定位到它们的目标器官.
  • 接触-FP对特别定位在附加器官之间的接口上.
  • 探测器可以可视化MCS的动态变化,包括对PLIN5过度表达的反应.
  • 实现了涉及单个器官细胞的多个MCS的同时可视化.

结论:

  • 接触FP为研究活细胞膜接触部位的动态和形态提供了一种多功能和强大的工具.
  • 这项技术克服了在可视化动态有机体相互作用方面以前的局限性.
  • 接触FP探头可以优化用于研究广泛的有机体对之间的MCS.