相关实验视频

Updated: Jul 4, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

12.0K

黄色和耐氧化衍生物的单体超级文件GFP的黄和耐氧化衍生物

Fernando M Valbuena, Adam H Krahn, Sherzod A Tokamov

    bioRxiv : the preprint server for biology
    |February 8, 2024
    PubMed
    概括

    研究人员开发了两种新的光蛋白 (FPs) 来自超级绿色光蛋白 (GFP). 这些优化的单体FP,msYFP2和moxGFP2,为生物成像提供了增强的光稳定性和细胞兼容性.

    更多相关视频

    Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast09:23

    Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

    Published on: November 28, 2018

    6.8K
    Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein06:10

    Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

    Published on: June 18, 2020

    7.3K

    相关概念视频

    Reporter Genes02:11

    Reporter Genes

    Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
    11.3K
    Tagging and Fusion Proteins01:24

    Tagging and Fusion Proteins

    Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
    6.6K