相关实验视频

Updated: Jul 4, 2025

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

使用多重受约束RNA速度模型的统计推断揭示了细胞周期速度调制

Alex R Lederer, Maxine Leonardi, Lorenzo Talamanca

bioRxiv : the preprint server for biology

|February 8, 2024

概括

VeloCycle为RNA速度推断提供了一个统计严格的框架,从单细胞RNA测序数据中改进了细胞周期动态和基因调节的分析.

科学领域:

计算生物学计算生物学
基因组学就是基因组学.
系统生物学系统生物学

背景情况:

细胞在生物过程中表现出协调的基因表达变化,形成低维的转录组动态.
单细胞RNA测序 (scRNA-seq) 捕获时间快照,而RNA速度估计基因表达动态使用结合和未结合的RNA.
现有的RNA速度算法缺乏统计的严格性和与基因表达多样性的动态一致性.

结论:

VeloCycle准确地推断细胞周期周期,并揭示基因特异性的动力学.
应用到体内和体外数据,VeloCycle确定了神经前体中的增殖模式和基因对细胞循环速度的淘汰效应.
VeloCycle 增强了 scRNA-seq 分析工具包,为RNA 速度推断提供了一个模块化和统计严格的框架.

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Measuring the Kinetics of mRNA Transcription in Single Living Cells

Measuring the Kinetics of mRNA Transcription in Single Living Cells

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相关概念视频

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...