营养消化的核心微生物群仍然存在,在经过连续批次培养肠微生物群in vitro后,氨的利用率增加
Mengyu Liu1, Tong Wang1, Lu Wang1
1College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
Frontiers in microbiology
|February 8, 2024
概括
使用高尿素的连续体外培养提高了肠微生物氨的利用效率. 保留了像Prevotella和Streptococcus这样的核心细菌,确定了在反动物中增强非蛋白质使用的生物标志物.
科学领域:
- 流体微生物学 流体微生物学
- 动物营养动物营养
- 在体外发酵技术发酵技术.
背景情况:
- 高尿素饮食可能会影响乳头功能和微生物组成.
- 了解尿素的利用对于有效的反动物营养至关重要.
- 实验室批量培养模型模拟皮状况,以研究这些影响.
研究的目的:
- 为了研究持续高尿素暴露对乳头消化功能的影响.
- 为了评估尿素利用能力的变化和乳头细菌组成.
- 为了识别与改善氨-N利用相关的细菌生物标志物.
主要方法:
- 在体外连续培养以23天的时间用5%的尿素培养液.
- 分析气体生产,干物质消化能力和细菌数量.
- 连续批量培养的反液 (CRF) 与新鲜的 (RF),冷的 (FRF) 和混合的 (MRF) 反液的比较.
主要成果:
- 干物质的消化能力保持稳定,但微生物的数量和多样性随着时间的推移而下降.
- 与射频,FRF和MRF相比,CRF显示出明显更高的氨-N利用效率.
- 保持了五个核心细菌属 (例如Prevotella,Streptococcus),并确定了特定的细菌作为氨利用的生物标志物.
结论:
- 在高尿素下持续的体外培养保留了核心菌并提高了氨-N利用效率.
- 鉴定了细菌生物标志物,为改善反动物的非蛋白质利用提供了见解.
- 这些发现支持优化反动物料效率和安全的潜在应用.
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