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通过液体染色学和质谱学测定定制的多2-基-2-索林的ω末端功能性

Nora Engel^1,2, Michael Dirauf^1,2,3, Justyna A Czaplewska^1,2

¹Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743 Jena, Germany.

Royal Society open science

|February 8, 2024

概括

这项研究引入了一种新的液体染色学方法,用于分析bicyclo[6.1.0]nonyne-functionalized poly(2-alkyl-2-oxazoline).该方法量化了对生物医学应用和点击化学至关重要的终端组修饰.

科学领域:

聚合物化学聚合物化学
分析化学分析化学
生物医学工程生物医学工程

背景情况:

先进的聚合物表征对于生物医学应用至关重要,特别是用于生物结合的隐形性质的聚合物.
对于针对特定反应设计的功能性聚合物来说,终端组分析至关重要,例如点击化学.

研究的目的:

开发和验证一种液态染色学 (LC) 协议,用于分析双环[6.1.0]非烯功能化多-2-基-2-zoline (POx).
为了能够定量确定POx.的欧米茄 (ω) 末端组的聚合后修饰.
提供首次报告的聚二甲基二氧化 (PMeOx) 的液体色谱分析.

主要方法:

聚二甲基二氧化 (PMeOx) 和聚二乙基二氧化 (PEtOx) 的合成,采用不同摩尔质量 (217.5 kDa) 的活离子环开放聚合 (CROP).
开发一种逆相液态色谱 (LC) 协议,使用C18衍生单质柱和乙和水的二进制梯度.
使用质谱测量分析化分数以确认终端组功能化和识别副产品.

主要成果:

成功合成PMeOx和PETOx与受控的质量.
建立一个强大的LC协议,用于对POx终端组转换进行定量分析.
通过LC-MS确认所需的 ω端组功能,并通过LC-MS识别合成杂质.

结论:

开发的LC协议对功能化POx,包括PMeOx的定量分析是有效的.
这种方法对于评估POx的质量和适用于生物医学应用和应变促进的点击反应的适用性至关重要.
这些发现提升了用于生物结合和药物输送系统的功能性聚合物的分析能力.

关键词:

电离环开放聚合 (CROP) 方法电喷离子质谱学 (ESI MS) 是一种电喷离子质谱学.液体色谱学液体色谱学液体色谱学矩阵辅助激光脱/离子化飞行时间质谱法 (MALDI-TOF MS)聚二氧化 (POx) 是一种聚二氧化.

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