通过全基因组诱导的遗传变异资源增强番茄功能基因组学
Prateek Gupta1,2, Pankaj Singh Dholaniya3, Kunnappady Princy1
1Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
Frontiers in plant science
|February 8, 2024
概括
132个以甲基硫酸盐 (EMS) 变异的番茄系的全基因组测序揭示了数百万种新型突变. 这个资源通过发现隐藏的遗传变异来加速番茄繁殖和功能基因组学.
科学领域:
- 植物遗传学 植物遗传学
- 基因组学就是基因组学.
- 农作物科学 农作物科学
背景情况:
- 诱导突变对于作物改进至关重要,为抗病和产量提供新的等位基因.
- 识别具有微妙或无可见表型的突变是具有挑战性的,但对于理解基因功能至关重要.
- 全基因组测序 (WGS) 提供了对变异性种群中所有突变的全面视图.
研究的目的:
- 综合识别和描述使用WGS的乙基甲硫酸盐 (EMS) 变异的番茄系中的诱导突变.
- 为功能性基因组学和番茄的有针对性的育种创造一个有价值的资源.
- 分析突变模式,包括对基因和代谢途径的偏差和影响.
主要方法:
- 测序132个双重EMS转基因化的西红线.
- 生物信息分析用于检测新突变和短插入/删除 (InDels).
- 使用从耐受性对不耐受性进行排序 (SIFT) 和对代谢途径进行映射来预测有害突变.
主要成果:
- 与家长品种相比,识别了大约4100万个新突变和550万个短 InDels.
- 在97%的基因组中发现了突变,包括基因,促进子,UTR和内子.
- 超过三分之一的基因含有有害突变,近四分之一影响多个代谢途径. 在典型的GC>AT偏差的同时,还观察到新的AT>GC过渡.
结论:
- 该研究产生了大规模的番茄突变数据库,显著增强了功能基因组学和育种的资源.
- 这些发现突显了WGS在发现广泛突变的实用性,包括那些具有非明显表型的突变.
- 验证的突变证明了发现管道的准确性,并提供了对番茄基因功能和代谢调节的见解.
关键词:
电子商务系统 (EMS) 是一个电子商务系统.这种植物是Solanum lycopersicum.在WGS中,使用的是WGS.codon 的使用情况功能性基因组学 功能性基因组学遗传代码是一种遗传代码.突变发生的突变发生.在这里,我们可以看到茄,番茄,番茄.更多相关视频
08:36Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
Published on: July 16, 2019
11.6K
11:15Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
Published on: November 9, 2011
22.9K
相关概念视频
03:21Genome Size and the Evolution of New Genes
8.0K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
8.0K
02:50Transgenic Plants
7.2K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.2K