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Updated: Jul 4, 2025

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
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Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

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无细胞基因组学:复制原始胚胎染色质中的转录因子相互作用

Peter B Becker1

  • 1Biomedical Center, Molecular Biology Division, Faculty of Medicine, LMU, Munich, Germany.

Biochemical Society transactions
|February 8, 2024
PubMed
概括

德罗斯菲拉前母细胞体胚胎提取物 (DREX) 创建动态色素用于研究转录因子结合. 这个系统揭示了蛋白质在复杂的染色质结构中的内在DNA结合特性.

关键词:
德洛索菲拉前母细胞皮胚胎.染色素免疫沉的免疫沉.核体结合的核体结合方式核酶体重塑的重塑开拓者的转录因子

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General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Factors02:16

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
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