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成年老鼠大脑中的Sonic Hedgehog转录的表征:与神经元和小质细胞标记物的共同表达
Mariagiovanna Russo1, Giuliana Pellegrino1, Hélène Faure1
1CNRS, Paris-Saclay University, UMR-9197, Neuroscience Paris-Saclay Institute, 91400, Saclay, France.
Brain structure & function
|February 8, 2024
概括
这项研究绘制了成年老鼠大脑中的Sonic Hedgehog (Shh) mRNA图,在各种神经元和寡细胞中发现表达,而不是天体细胞. 亚基神经元是SHH可用性的关键调节者.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 天体细胞被认为是成年哺乳动物大脑中主要的Sonic Hedgehog (Shh) 反应细胞.
- 对于通路激活至关重要的SHH连接体的细胞来源在大脑中仍然定义不佳.
研究的目的:
- 在成年小鼠大脑中全面地绘制Shh mRNA表达细胞的分布和细胞表型.
- 澄清负责产生SHH的细胞类型,从而为了解SHH信号功能提供信息.
主要方法:
- 单分子光在位杂交 (smfISH) 用于检测Shh mRNA转录.
- 免疫组织化学用于识别表达Shh mRNA的特定细胞群,包括神经元和质标记物.
主要成果:
- Shh mRNA在大脑区域的表达范围比以前报告的更广泛.
- 在多种神经元亚型 (GABAergic,胆能,多巴胺能,能,谷氨酸) 和寡类细胞的一个子集中确定了Shh转录.
- 在星体细胞 (S100β+) 和微质细胞 (Iba1+) 中,SHH表达显著缺失.
结论:
- 这项研究提供了迄今为止最详细的中央神经系统中SHH表达细胞的地图.
- 氧化合成酶 (nNOS) 表达神经元在调节Shh可用性方面发挥着重要作用.
- 这些发现为研究Shh信号在大脑功能和疾病中的作用奠定了基础.
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02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...