缺氧增加了IGF2BP3结合的圆形RNAs的生物发生
Kriti Kaushik1, Hemant Kumar1, Samriddhi Mehta2
1Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, Convergence Block, New Delhi, 110029, India.
Molecular biology reports
|February 8, 2024
概括
缺氧会增加循环RNA (circRNA) 的产生,可能导致瘤的进展. 这项研究研究了胰岛素样生长因子2结合蛋白3 (IGF2BP3) 和 (QKI) 在缺氧诱导的circRNA生物发生中的作用.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 在RNA生物学,RNA生物学.
背景情况:
- 胰岛素样生长因子2结合蛋白3 (IGF2BP3) 通过与各种RNAs相互作用,与癌症进展有关.
- 缺氧是瘤中常见的特征,可调节缺氧诱导因素并促进瘤生长.
- 由低氧诱导的Quaking (QKI) 基因增强了表皮细胞到介质细胞的过渡 (EMT) 和循环RNA (circRNA) 生产.
研究的目的:
- 在低氧条件下研究IGF2BP3,QKI,circRNA及其宿主基因之间的轴.
- 了解IGF2BP3和QKI在缺氧诱导的circRNA生物发生中的作用及其对瘤进展的贡献.
主要方法:
- 使用定量实时PCR (qRT-PCR) 来评估IGF2BP3,QKI,EMT标记物,circRNA和宿主mRNA的表达.
- RNA免疫沉 (RIP) 测定证实了IGF2BP3与特定circRNAs的结合.
- 细胞系 (HeLa,HepG2,U87MG) 在正常和缺氧条件下进行培养,以分析基因表达变化.
主要成果:
- 缺氧高调低氧标志物 (VEGF,CA9) 和所有细胞系中的SNAIL.
- 在缺氧下,IGF2BP3和QKI的表达增加,这表明它们参与circRNA生物发生和稳定.
- 六个宿主基因 (PHC3,CDYL,ANKRD17,ARID1A,NEIL3,FNDC3B) 在低氧状态下显示mRNA和circRNA的表达增加,这表明在瘤启动中具有协同作用.
- 在缺氧下,大多数基因和时间点的circRNA与mRNA表达比率增加.
结论:
- 缺氧可以增强circRNA生物发生.
- 在缺氧下观察到的circRNA产量的增加可能会导致瘤的进展.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
02:57piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:13Formation of Muscle Fibers from Myoblasts
4.9K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.9K
02:55The Nucleolus
8.8K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.8K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K