一个基于光的协议,用于定量定位CUT&RUN缓冲元件的缓冲元件

相关实验视频

Updated: May 10, 2026

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
11:01

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies

Published on: November 20, 2014

51.2K

Andrew Katznelson1, Kenneth Zaret1

  • 1Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

STAR protocols
|February 8, 2024
PubMed
概括

这项研究引入了一种基于光的方法,以优化在目标下切割和释放,使用核酶 (CUT&RUN) 缓冲条件. 该协议确保了有效的细胞透和表皮质保留,以便进行准确的基因组分析.

关键词:
细胞生物学 细胞生物学基于细胞的测试在 ChIP-seqq.染色体免疫沉 (ChIP) 是一种遗传学 遗传学 是一个分子生物学分子生物学

更多相关视频

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues06:54

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

Published on: June 3, 2021

4.3K
Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells14:25

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Published on: May 13, 2022

6.7K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...