在地表水中检测沙门氏菌菌体,作为环境监测的可扩展方法
Sneha Shrestha1,2, Kesia Esther Da Silva3, Jivan Shakya4
1Center for Infectious Disease Research and Surveillance, Dhulikhel Hospital Kathmandu University Hospital, Kavre, Nepal.
PLoS neglected tropical diseases
|February 8, 2024
概括
沙门氏菌 Typhi 菌在伤寒流行地区的地表水中丰富,为环境监测提供了低成本的方法. 这些菌体是S. Typhi特有的,并且需要其Vi多糖化物来感染.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 公共卫生 公共卫生
背景情况:
- 对沙门氏菌Typhi (S. Typhi) DNA的环境监测是识别台风流行环境的工具,但成本昂贵,需要分子诊断.
- 该研究探讨了水源中S Typhi菌的丰富性,作为监测的潜在低成本替代方案.
研究的目的:
- 为了确定在风疹流行地区内的水源中S. Typhi菌的丰富性.
- 评估使用低成本菌体检测试验用于伤寒监测的可行性.
主要方法:
- 从尼泊尔的城市,郊区和农村地区采集了水样 (饮用水和地表水).
- 使用S. Typhi的双甲基覆盖试验用于检测细菌菌体.
- 孤立的菌体对宿主范围进行了测试,并进行了全基因组测序以进行遗传学分析.
主要成果:
- 在54.9%的河水样本中检测到S. Typhi特异性的菌体,但很少在低发病率地区发现.
- 菌体存在与人口密集地区的邻近相关.
- 隔离的菌体是S. Typhi特有的,并且需要Vi多糖化物来感染,聚合成三个不同的基因组.
结论:
- 伤寒菌在伤寒流行社区的地表水中大量存在,这表明它们对环境监测的潜力.
- 用于S. Typhi菌体检测的低成本斑块检测是一种可扩展和具有成本效益的替代DNA方法.
- 建议对这种基于菌体的方法进行进一步评估,以对伤寒环境进行监测.
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