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血微生物无细胞DNA测序用于在患有肌肉骨感染的儿童中检测和量化病原体

James B Wood^1,2, Kelsey Russell¹, Tom E Davis³

¹Ryan White Center for Pediatric Infectious Diseases and Global Health, Indiana University School of Medicine, Indianapolis, Indiana, USA.

Journal of the Pediatric Infectious Diseases Society

|February 8, 2024

概括

血微生物无细胞DNA (mcfDNA) 测序显示出在儿科肌肉骨感染 (MSKI) 中识别病原体的前景,特别是在手术前采集样本时. 这种方法提供了比传统血液培养更长的检测窗口.

科学领域:

分子诊断学分子诊断
儿童传染病儿童传染病
基因组医学是基因组医学.

背景情况:

儿科肌肉骨感染 (MSKIs) 经常表现为培养负,限制了准确的诊断.
血微生物无细胞DNA (mcfDNA) 测序为培养提供了一个非侵入性的替代方案,绕过了常见的诊断障碍.

研究的目的:

在儿科MSKI中评估血mcfDNA测序的诊断性能.
为了将mcfDNA测序的病原体检测时间与传统的血液培养进行比较.
评估样本时间 (手术前与手术后) 对mcfDNA测序一致性的影响.

主要方法:

从2019年7月到2022年5月,对儿童 (6个月18岁) 与MSKIs进行前性研究.
收集了连续的血mcfDNA测序样本,并与同时进行的血液培养进行了比较.
分析包括病原体恢复率,一致性和检测持续时间.

主要成果:

在81%的病例中,mcfDNA测序确定了病原体,而在培养过程中,这一比例为68%.
当手术前 (82%) 和手术后 (20%) 采集样本时,与培养物的一致性更高.
mcfDNA测序检测到病原体的时间比血液培养时间 (平均1天) 长得多 (平均2天).

结论:

血mcfDNA测序是诊断培养负的儿科MSKI的一个有价值的工具,特别是在手术前采集样本时.
频繁检测多种病原体需要仔细解释和诊断管理.
与血液培养相比,mcfDNA测序扩大了病原体检测的窗口.

关键词:

诊断诊断诊断诊断诊断微生物无细胞DNA测序肌肉骨系统感染骨质炎是一种骨质炎.儿科儿科儿科儿科败血性关节炎是什么意思

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