泌尿病原性大肠杆菌 (Escherichia coli) 颠覆宿主自防御系统,通过阻断自前结构来逃脱溶解体外细胞分解
Xueping Li1,2, Lingyan Jiang1,2, Si Zhang1,2,3
1Tianjin Economic-Technological Development Area Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, People's Republic of China.
The Journal of infectious diseases
|February 8, 2024
概括
泌尿病原性大肠杆菌 (UPEC) 使用脂酶PldA抑制自,防止溶酶体外细胞化并促进尿路感染. 这种机制使UPEC能够形成细胞内细菌群落以求生存.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 尿路感染 (UTI) 通常是由尿病原性大肠杆菌 (UPEC) 引起的.
- UPEC侵入膀上皮细胞 (BECs),形成细胞内细菌群落 (IBCs),并逃避宿主免疫反应.
- 通过UPEC逃避自并建立IBC的机制尚未完全理解.
研究的目的:
- 阐明UPEC外膜脂酶PldA在宿主细胞内的细菌生存中的作用.
- 调查UPEC PldA如何干扰细胞过程,如自和溶酶体外细胞分裂.
- 了解PldA对急性尿路感染病原体的贡献.
主要方法:
- 研究了UPEC与BECs的相互作用.
- 分析了PldA在细菌入侵和细胞内生存中的作用.
- 评估了PldA对自流和溶酶体外细胞的影响.
- 测量了细胞内酸-3酸盐水平和NDP52的招募.
主要成果:
- UPEC PldA 抑制了 BEC 中的自流,减少了溶酶体外细胞.
- PldA可以降低细胞内酸-3-酸盐的水平.
- 这导致NDP52颗粒积累增加和NDP52对自的向受损,阻碍了前自细胞形成.
- UPEC PldA促进细菌从宿主细胞防御中逃脱,促进尿路感染.
结论:
- UPEC PldA在抑制宿主细胞内的自流中发挥着至关重要的作用.
- 通过破坏自和 lysosome exocytosis,PldA促进了UPEC的生存和细胞内细菌群落的形成.
- 这些发现凸显了PldA作为UPEC病原体的关键因素和尿路感染治疗的潜在目标.
更多相关视频
相关概念视频
Delivery Pathways to the Lysosome
6.5K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.5K
Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
Export of Misfolded Proteins out of the ER
3.6K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.6K
Recycling Endosomes and Transcytosis
2.7K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.7K
Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K
The Proteasome
835
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
835


