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Jun Yang¹, Gokhan Bahcecioglu², George Ronan³

¹Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA.

|February 8, 2024

概括

衰老的乳腺组织微环境,特别是矩阵结合囊泡 (MBVs),促进乳腺癌. 陈旧的MBV含有促进癌症的因素,增加细胞运动和入侵,突出显示它们在与衰老相关的乳腺癌进展中的作用.

科学领域:

生物医学科学生物医学科学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

老龄化是乳腺癌的一个重要风险因素.
虽然研究了细胞衰老的影响,但衰老的乳腺组织微环境,特别是细胞外基质 (ECM),仍未得到充分探索.
矩阵结合囊泡 (MBVs) 是乳腺ECM中新发现的成分,具有潜在的调节作用.

研究的目的:

在年轻与老年健康小鼠乳腺组织中识别和表征矩阵结合囊泡 (MBVs).
研究不同年龄组的MBV对正常和癌症人类乳腺上皮细胞的影响.
阐明MBVs在与年龄相关的乳腺癌进展和转移中的作用.

主要方法:

在年轻和老老的小鼠乳腺组织中识别和描述MBVs.
囊泡标记技术可视化从脱细胞化组织中MBVs的细胞吸收.
对MBVs的miRNA和细胞因子分析.
使用正常 (KTB21) 和癌症 (MDA-MB-231) 乳腺细胞进行的细胞培养实验.

主要成果:

在健康小鼠的乳房ECM中发现了MBV,并被证明被乳腺细胞内化.
从老年小鼠中分离出来的MBVs表现出一种转向更具瘤性和侵入性的表型的转变.
与年轻的MBV相比,老年MBV显示癌症相关的细胞因子和瘤反应因子 (miR-10b,miR-30e,miR-210) 的增加.
用老化MBV或其丰富的miRNAs/AdipoRon主动剂治疗显著增加了细胞移动性和侵入性.

结论:

在组织微环境中,MBVs起着调节作用.
随着年龄的增长,MBV的组成会发生变化,从而促进了瘤原生环境.
了解MBV的功能和货物对于了解老年人群中乳腺癌的进展和转移至关重要.

关键词:

衰老的衰老衰老的衰老乳腺癌乳腺癌乳腺癌脱细胞化细胞外基质 (dECM)这是入侵.矩阵结合囊泡 (MBVs) 是一种移动性移动性移动性

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