通过piRNA-DQ717867/p53通路破坏精子生成细胞循环
Jiaoyang Wei1, Juan Dai2, Xiaofan Shi3
1School of Public Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, China.
Reproductive toxicology (Elmsford, N.Y.)
|February 8, 2024
概括
暴露于会通过改变piRNA表达来破坏雄性大鼠的精子生成. 具体来说,piRNA-DQ717867可能调节p53,导致细胞循环停止和生殖毒性.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- (Cd) 是一种有毒的环境污染物,影响多个器官系统.
- 男性生殖毒性是与暴露相关的重大问题.
- 皮维相互作用RNAs (piRNAs) 在精子生成和基因组稳定性中起着至关重要的作用.
研究的目的:
- 为了研究化 (CdCl2) 暴露对雄性大鼠精子生成的影响.
- 确定受暴露影响的特定piRNA,并阐明它们在生殖毒性中的作用.
- 探索诱导的piRNA失调影响细胞周期控制的机制.
主要方法:
- 雄性大鼠被口服CdCl2暴露了28天.
- 用微阵列和qPCR分析丸组织以检测piRNA的表达.
- 用GC-2spd细胞研究piRNA操纵对细胞周期调节的影响.
主要成果:
- 暴露于会显著改变大鼠丸中的piRNA表达,特别是piRNA-DQ759395.5.
- 一种类似的piRNA,piRNA-DQ717867,在CdCl2暴露后在GC-2spd细胞中被上调,导致细胞循环停止.
- 有证据表明,piRNA-DQ717867与p53相互作用,破坏细胞循环相关的蛋白质表达,并激活p53通路.
结论:
- 暴露于会诱导大鼠丸中的piRNA表达异常,导致雄性生殖毒性.
- piRNA-DQ717867在调节p53和GC-2spd细胞中的细胞周期进展方面发挥作用.
- 这些发现凸显了piRNAs在维持男性生殖健康和减轻引起的毒性方面的关键作用.
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