改变离子强度可以调节包括粉和蛋白质或其解剂在内的二元复合物的耐性粉含量
Zexue Lin1, Yanyu Chen1, Gaolei Xi2
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; College of Food Science, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Southwest University, Chongqing 400715, China.
International journal of biological macromolecules
|February 8, 2024
概括
增强粉-蛋白质复合物的离子强度放松了结构,增强了粉的消化. 大豆蛋白水解剂最初会增加耐性粉,但由于食品加工中离子强度更高,这种效果会降低.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 离子强度是食品加工的关键因素,影响分子相互作用.
- 不同离子强度对粉-蛋白质复合物的结构和消化能力的影响尚不清楚.
- 了解这些相互作用对于优化食品产品开发和营养特性至关重要.
研究的目的:
- 研究不同离子强度如何影响由原生玉米粉和大豆蛋白分离物/水解物形成的二元复合物的结构.
- 确定离子强度对这些粉蛋白复合物的消化能力和耐性粉含量的影响.
- 阐明蛋白质类型 (分离物与水解物) 和离子强度对复杂结构和粉可访问性的联合影响.
主要方法:
- 用原生玉米粉 (NS) 和大豆蛋白分离物 (SPI) 或大豆蛋白水解物 (SPIH) 在不同NaCl度 (0-0.40M) 中制备二元复合物.
- 进行结构分析,观察网络形成和分子组织的变化.
- 通过测量耐性粉 (RS) 含量来评估消化性,这表明粉对粉酶的可访问性程度.
主要成果:
- SPI和SPIH都与NS形成了紧的网络结构,SPIH由于分子尺寸减少而产生了更丰富的耐性粉 (RS) 含量.
- 增加的离子强度导致了更大的非周期性结构和复合体内的松散网络形成.
- 较高的离子强度通过提高粉酶的可访问性,显著提高了粉的消化能力,从而使RS含量从19.07%降至15.52%.
结论:
- 豆蛋白水解剂可以增加耐性粉,但通过增加离子强度来抵消这种效应.
- 较高的离子强度通过松复杂的结构来促进粉的消化,这是主食生产的关键考虑因素.
- 必须管理蛋白质类型和离子强度之间的相互作用,以控制加工食品中的粉消化能力和RS形成.
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