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在白中通过嗅觉共受体Orco调节食行为的神经调节

Huan Xu¹, Yongyong Gao¹, Ali Hassan²

¹Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Research and Development Centre of Ecological and Sustainable Application of Microbial Industry of the Loess Plateau in Shaanxi Province, Yan'an University, Yan'an 716000, Shaanxi, China; Key Laboratory of Termite Control of Ministry of Water Resources, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

International journal of biological macromolecules

|February 8, 2024

概括

神经和嗅觉共受体 (Orco) 对于虫的食至关重要. 沉默这些基因会损害嗅觉识别,并减少食成功,突出显示它们在生存行为中的关键作用.

科学领域:

昆虫神经生物学昆虫神经生物学
化学生态化学生态学
行为生态学行为生态学

背景情况:

嗅觉对于动物的生存至关重要,指导食和社会互动.
已知神经可以调节昆虫的嗅觉和行为.
在食甲中嗅觉识别的特定神经调节仍然不太了解.

研究的目的:

研究嗅觉共受体基因 (Orco) 和神经Y基因 (NPY) 在白嗅觉识别和食行为中的作用.
为了确定Orco和NPY沉默对*Odontotermes formosanus*在不同掠食压力下食成功的影响.
探索在食期间对掠食者的社会缓冲的潜力.

主要方法:

通过双链RNA (dsRNA) 注射基因沉默,以工作中的Orco和NPY为目标.
在天线和电磁天线 (EAG) 响应轨道激素的Orco蛋白表达的分析.
评估行走活动,食成功,以及在模拟掠食压力下的行为反应.
坐标实验验证了Orco和NPY的作用.

主要成果:

淘汰Orco减少了Orco蛋白的表达和EAG对痕迹激素的反应.
通过降低Orco表达的调节,NPY沉默损害了嗅觉反应.
对Orco和NPY的沉默显著降低了步行活动和食成功.
足迹激素和巢同伴士兵提供了社会缓冲,防止掠食者对食的影响.

结论:

奥尔科和NPY是操纵白在食期间嗅觉识别的重要基因.
嗅觉识别的神经调节在控制白的食行为中起着至关重要的作用,特别是在捕食风险下.
社交线索可以减轻掠食对有完整的嗅觉能力的白的食行为的负面影响.

关键词:

食行为是寻找食物的行为.神经酸Y 神经酸Y 嗅觉识别是一种嗅觉识别.

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