在同一接口上检测微RNA的SERS电化学双模式检测,辅助由外核酶III信号转换

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Updated: Jul 4, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Chaoqin He¹, Yali Hu¹, HaoPeng Qi¹

¹Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.

Analytica chimica acta

|February 8, 2024

概括

这项研究引入了一种新的双模式生物传感器,用于检测microRNA 21. 这种创新方法结合了表面增强拉曼光谱 (SERS) 和电化学方法,用于增强癌症生物标志物检测.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
分子诊断学分子诊断

背景情况:

双模式传感为检测癌症生物标志物提供了更高的准确性和可靠性.
微RNA 21是一种重要的生物标志物,与各种癌症有关.
现有的检测方法在灵敏度或特异性方面可能存在局限性.

研究的目的:

开发一种新的表面增强拉曼光谱 (SERS) 和电化学双模式生物传感器.
使用外核酶III辅助信号转换检测癌症生物标志物microRNA 21.
为微RNA检测建立一个敏感和可靠的平台.

主要方法:

在电极上使用金纳米粒子 (Au NPs) 制造SERS基板.
用Mn3O4/DNA链对基质进行修改,用于初始电化学信号生成.
使用外核酶III (Exo III) 在微RNA21结合时触发信号转换,使SERS检测和电化学信号减少.

主要成果:

生物传感器展示了对microRNA 21的双模式检测能力.
在SERS的低检测极限 (LOD) 达到3.98 × 10−3 nM.
在电化学检测中实现了6.89 × 10−5 nM的低LOD.
显示了两种传感模式的良好线性校准曲线.

结论:

开发的生物传感器在单个接口上为微RNA提供了巧妙的双检测模式.
这项工作提出了SERS和电化学分析的结合新策略.
双模式生物传感器显示了准确可靠的癌症生物标志物检测的潜力.

关键词:

双模式双模式双模式电化学电化学电化学异核酶III可以产生异核酶.这是一个微型RNA.这就是 SERS SERS.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...