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通过缺氧MoO_3-x对α-葡萄糖酶进行多酶级联分析

Fengxian Zhang¹, Jiawei Liu¹, Zhi Chen¹

¹National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, College of Health Science and Engineering, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, PR China.

Analytica chimica acta

|February 8, 2024

概括

新型缺氧三氧化纳米材料为酶级联分析提供了一个敏感而稳定的平台. 这种对H2O2敏感的材料能够准确检测alpha-glucosidase活性和抑制剂选.

科学领域:

材料科学材料科学材料科学
生物化学生物化学
分析化学分析化学

背景情况:

酶级联反应对于生物传感器的发展至关重要,通常依赖过氧化酶 (POD) /氧化酶系统.
现有的POD/氧化酶系统由于恶劣的酸性条件,不良稳定性和氧化酶干扰而面临限制.
需要新的H2O2敏感色素纳米材料来代替POD纳米酶在酶级联生物分析中.

研究的目的:

为H2O2敏感应用开发和利用新型缺氧MoO3-x纳米材料.
在酶级联测定中使用这些纳米材料进行α-Glucosidase活性分析.
为了证明这种测试对选α-葡萄糖酶抑制剂的有用性.

主要方法:

缺氧MoO3-x的超快速合成,具有H2O2敏感性和近红外 (NIR) 吸收.
酶级联反应涉及α-Glucosidase和葡萄糖氧化酶 (GOx).
在770nm的NIR吸收变化的色度检测检测.

主要成果:

合成的MoO3-x表现出H2O2的敏感性和NIR吸收.
酶级联测定成功检测出α-葡萄糖酶活性,检测极限为8 x 10^-5 U/mL.
该试验经过抑制剂查的验证,并通过便携式套件适应智能手机和微板阅读器分析.

结论:

基于MoO3-x的酶级联测定是一种有前途的生物分析工具,涉及H2O2的产生或消耗.
这一战略推动了对H2O2敏感的染色化纳米材料的开发,用于生物分子和酶分析.
开发的便携式套件提供了一种方便的方法来检测alpha-glucosidase活动.

关键词:

测色仪检测检测的颜色测量检测.多酶级联是多种酶的级联.缺乏氧气的MoO{3-x) 的情况.α-葡萄糖化酶的使用.

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