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在全基因组序列分析中识别知识缺口的多耐药耐热Campylobacter spp

Michael Zarske¹, Huong Quynh Luu², Carlus Deneke¹

¹Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, Berlin, D-12277, Germany.

|February 8, 2024

概括

全基因组测序揭示了坎比洛巴克特的频繁,未报告的耐药性基因变异,包括重复和突变,挑战全球食品安全. 需要进一步的研究,以了解新的抵抗机制和传播.

科学领域:

微生物学微生物学
基因组学就是基因组学.
食品安全食品安全

背景情况:

坎比洛巴克特 spp. 的种类. 是导致细菌性食源性胃肠炎的主要原因.
它被世界卫生组织认定为高优先级的抗生素耐药细菌.
目前的欧洲监测低估了坎皮洛巴克特抗生素耐药性的全球负担.

研究的目的:

系统地比较全基因组测序 (WGS) 与表型抗微生物敏感性测试.
分析来自越南和德国的热耐药Campylobacter家禽分离物中的抗生素耐药性 (AMR).
为了识别异常,并改进抗菌素耐药性基因的生物信息分析.

主要方法:

系统地比较WGS和表型抗微生物敏感性测试.
分析了494个耐热的Campylobacter家禽分离物.
利用长期以来的牛津纳米孔技术进行闭性染色体和等离子体分析.

主要成果:

在三个基因 (gyrA,23S rRNA,rpsL) 中确定了22个耐药基因/变异和点突变.
观察到频繁的耐药性基因重复,马赛克主义和新突变,包括敏感菌株中的型变异.
由于重复,同质性或相变化,在识别某些基因 (tet(O), aadE, aad9) 中存在短读序列限制.

结论:

常见的,未报告的耐药性基因变异 (重复,马赛克,突变) 在Campylobacter中存在.
现有的数据库缺少关键的抵抗信息.
坎皮洛巴克特的未知耐药机制和传播构成了全球粮食安全的重大挑战.

关键词:

抗菌药物反症 (AMR) 是一种抗生素耐药性抗生素耐药性长读序列的测序方式马赛克基因马赛克基因多种药物耐药性的岛屿没有NGS,没有NGS.阻力监测阻力监测阻力监测阻力监测东南亚的东南亚地区.敏感性测试测试敏感性测试

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