全转网红酸减轻了感染性胃肠炎病毒引起的肠道炎症和断小猪的屏障功能障碍
Junning Pu1,2, Daiwen Chen1,2, Gang Tian1,2
1Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People's Republic of China.
Journal of animal science and biotechnology
|February 8, 2024
概括
全跨网红酸 (ATRA) 补充剂改善了感染性胃肠炎病毒 (TGEV) 的小猪的生长,减少了感染性胃肠炎病毒 (TGEV) 的小猪的腹. 通过抑制NF-κB信号通路,ATRA增强了肠道屏障功能并减少了炎症.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 传染性胃肠炎病毒 (TGEV) 在小猪中引起显著的腹和肠炎症.
- 全转录氨酸 (ATRA) 是维生素A的代谢物,具有已知的免疫调节和抗炎性质.
- 在小猪中减轻TGEV诱导的肠损伤方面,ATRA的疗效在很大程度上仍未被探索.
研究的目的:
- 研究ATRA补充剂对TGEV挑战性猪仔生长表现,腹发病率和肠道健康的影响.
- 阐明ATRA对肠道屏障完整性和TGEV感染后的炎症反应的影响.
- 探索潜在的分子机制,包括信号通路,通过ATRA可能产生保护作用.
主要方法:
- 一项为期19天的研究涉及32只被断奶的猪仔,这些猪仔被随机分配到四组:对照组,TGEV挑战组,TGEV+5 mg/dATRA组和TGEV+15 mg/dATRA组.
- 在第14天,TGEV挑战是口服的.
- 评估了生长性能,腹得分,血清二胺氧化酶 (DAO) 活性,肠壁蛋白水平 (occludin,claudin-1),酶活性 (sucrase),基因表达,炎症性细胞因子水平和信号通路组件.
主要成果:
- 补充ATRA (5和15毫克/天) 显著缓解了TGEV挑战性猪仔的生长抑制和腹.
- 通过使血清DAO活性正常化,以及内奥克卢丁和克劳丁-1蛋白水平,ATRA保持了肠道屏障的完整性.
- 亚特拉 (5毫克/天) 增强了糖酶活性和营养载体基因表达 (GLUT2,SLC7A1),减弱了炎症性细胞因子释放 (IL-1β,IL-8,TNF-α),并促进了抗炎媒介 (IL-10,sIgA).
- 亚特拉降低了关键炎症通路基因 (TLR3,TLR4,RIG-I,MyD88,TRIF,MAVS) 和NF-κB信号传递 (减少了p-NF-κB p65,增加了IκBα).
结论:
- ATRA有效地减轻TGEV诱导的肠壁损伤,并改善小猪的生长性能和腹.
- 保护机制包括抑制炎症反应,特别是通过由TLR3,TLR4和RIG-I.调节的NF-κB信号通路.
- 亚特拉显示出作为治疗猪TGEV感染管理的治疗剂的潜力.
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