对肠道菌群与结直肠癌患者的不同BMI状态相关的肠道菌群差异的分析
Yongqi Huang1, Xiaoliang Huang1, Zhen Wang1
1Division of Colorectal and Anal Surgery, Department of Gastrointestinal Surgery, Guangxi Medical University Cancer Hospital, Nanning, People's Republic of China.
Journal of translational medicine
|February 8, 2024
概括
超重的结直肠癌 (CRC) 患者表现出明显的肠道细菌,包括Actinomyces和Bacteroides,可能会影响瘤免疫力. 这突显了体质指数 (BMI) 和肠道微生物组在CRC中的联系.
科学领域:
- 微生物组研究的研究.
- 胃肠病学 胃肠病学
- 在瘤学瘤学.
背景情况:
- 超重是结直肠癌 (CRC) 的重要危险因素.
- 肠道微生物组在CRC中的作用因体重指数 (BMI) 而有所不同,但仍然不完全理解.
- 正常体重和超重CRC患者之间的肠道菌群组成和功能差异需要阐明.
研究的目的:
- 调查不同BMI状态的CRC患者肠道菌群的丰度,组成和生物功能的差异.
- 探索BMI分层肠道微生物群与CRC中的瘤免疫微环境之间的关系.
主要方法:
- 170名CRC患者被分为超重 (BMI ≥24 kg/m2) 和正常体重 (BMI 18.5-23.9 kg/m2) 的两组.
- 进行了便样本的16S rRNA测序和瘤组织的转录组测序.
- CIBERSORT算法分析了免疫细胞的组成,并评估了微生物群,免疫细胞和基因之间的相关性.
主要成果:
- 两组之间没有观察到肠道微生物群的α和β多样性的显著差异.
- 部分最小平方差分分析 (PLS-DA) 基于BMI分层区分了植物群.
- 确定了33种不同丰富的种类;在超重组中,Actinomyces,Desulfovibrio和Bacteroides被丰富.
- "其他类型的O-甘氨酸生物合成"途径在超重组中得到了丰富.
- 克洛斯特里IV与M2巨细胞和调节性T细胞 (Tregs) 有正相关,与激活的树突细胞和CD4记忆性T细胞有负相关.
结论:
- 在CRC患者中,肠道微生物群的丰富性和多样性可能与BMI状态相关.
- 像Actinomyces,Desulfovibrio和Bacteroides这样的特定细菌可能与CRC患者的超重状况有关.
- 瘤微环境表现出独特的免疫景观,受到BMI相关的差异性菌群的影响,这表明CRC通过免疫调节在CRC病变发生过程中发挥了作用.
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