将循环神经网络连接到SPAD TCSPC系统,用于实时光寿命成像
Yang Lin1, Paul Mos1, Andrei Ardelean1
1Advanced Quantum Architecture Laboratory, École polytechnique fédérale de Lausanne, Neuchâtel, 2002, Switzerland.
Scientific reports
|February 8, 2024
概括
边缘人工智能通过循环神经网络 (RNN) 增强光终身成像 (FLI),使医学诊断更快,更准确. 这种强大的方法直接处理数据,改善实时成像能力.
科学领域:
- 生物医学工程 生物医学工程
- 人工智能在医学中的应用
- 光子学 是一个光子学.
背景情况:
- 光终身成像 (FLI) 是生物学和医学中一个有价值的诊断工具.
- 目前的FLI系统在速度,准确性和稳定性方面面临限制.
- 边缘人工智能 (Edge AI) 为这些挑战提供了潜在的解决方案.
研究的目的:
- 使用Edge AI开发一种强大而快速的FLI方法.
- 为了实现实时的FLI采集,而不会降低准确度.
- 为了减少FLI系统中的数据传输量和硬件资源利用量.
主要方法:
- 将循环神经网络 (RNN),专门封闭的循环单元 (GRU) 和长短期记忆 (LSTM) 连接到SPAD TCSPC系统.
- 训练RNN从原始时间直接估计光寿命,绕过直方图结构.
- 在FPGA上实现量子化GRU核心,用于实时数据处理.
主要成果:
- 基于RNN的FLI表现出与传统方法 (CMM,LS装配) 相比的准确性.
- 在存在背景噪音的情况下,RNN显著优于传统方法.
- 导出的克拉默-拉奥下界表示RNN寿命估计的近最佳精度.
- 一个实时FLI显微镜达到每秒10.
结论:
- 拟议的Edge AI驱动的FLI系统为生物医学应用提供了强大,快速和准确的解决方案.
- 这种方法显著提高了用于诊断和研究的实时成像能力.
- 该系统非常适合用于生物成像,医学诊断和光辅助手术.
更多相关视频
07:41Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
Published on: July 19, 2016
7.7K
10:41Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
Published on: June 7, 2019
8.5K
相关概念视频
01:37Super-resolution Fluorescence Microscopy
7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K