长非编码RNA SNHG1通过调节miR-641/RRS1轴来促进乳腺癌的进展
Lin Deng1,2, Jun Wang3,4,5, Junying Song1
1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Qingdao University, Qingdao, 266071, China.
Scientific reports
|February 8, 2024
概括
小核核RNA宿主基因1 (SNHG1) 通过抑制miR-641和上调RS1.1,促进乳腺癌 (BC) 的进展. 下调SNHG1抑制BC生长和转移,这表明SNHG1是潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地与癌症的发展有关.
- 乳腺癌 (BC) 进展中 lncRNAs 的特定分子机制尚未完全理解.
研究的目的:
- 研究小核核RNA宿主基因1 (SNHG1) 在乳腺癌进展中的作用.
- 阐明在BC中SNHG1的潜在分子机制.
主要方法:
- 定量实时PCR测量BC组织和细胞中的SNHG1表达.
- 细胞增殖,迁移,入侵和亡的测试.
- 双露西法酶记者基因测试和RNA拉下测试以确定分子相互作用.
- 在体内瘤异种移植模型评估SNHG1对BC生长的影响.
主要成果:
- 在BC组织和细胞中,SNHG1被显著上调.
- 降低SNHG1的调控抑制了BC细胞的增殖,迁移,入侵和上皮细胞-介质细胞过渡 (EMT),同时促进了细胞亡.
- SNHG1直接针对miR-641,这反过来又针对RRS1.
- 在体内研究证实,SNHG1下调抑制BC瘤生长.
- SNHG1通过抑制miR-641并因此上调RRS1.1,促进BC进展.
结论:
- 在促进乳腺癌进展方面,SNHG1起着至关重要的作用.
- SNHG1/miR-641/RRS1轴是BC发育的一个关键途径.
- SNHG1代表了乳腺癌治疗的潜在治疗标.
相关概念视频
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
01:23Negative Regulator Molecules
35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K