相关实验视频

Updated: Jul 4, 2025

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

人类原型多能干细胞通过内源性BMP5/7诱导分化为 trofhoblast干细胞,而不经过天真状态的过渡

Ethan Tietze¹, Andre Rocha Barbosa^1,2,3, Bruno Araujo¹

¹Lieber Institute for Brain Development, Baltimore, MD, USA.

Scientific reports

|February 9, 2024

概括

人类多能干细胞 (hPSCs) 可以通过原始状态分化为热囊细胞干细胞 (TSCs). 这个过程激活了特定的通路,并抑制了其他通路,产生了功能性的胎盘细胞,而没有外源性骨形态遗传蛋白质.

科学领域:

干细胞生物学干细胞生物学
发育生物学是发展生物学.
胎盘生物学胎盘生物学

背景情况:

人类热细胞干细胞 (TSC) 在试验室中对胎盘发育的建模至关重要.
人类多能干细胞 (hPSC) 到TSC的分化途径尚未完全理解.
从hPSC生成TSC的有效方法的识别对研究至关重要.

研究的目的:

研究原始化hPSCs转化为TSCs的分化.
为了确定参与这种差异化过程的信号通路.
描述由此产生的TSC及其进一步差异化的潜力.

主要方法:

时间单细胞RNA测序用于分析不同条件下的分化.
差异化协议的比较,包括定义的"TS条件" (EGF,WNT激活;TGFβ,HDAC,ROCK抑制) 与依赖BMP4的协议.
细胞群体的特征化,以鉴定热囊细胞分化和多能性的标记.

主要成果:

"TS条件"成功地从没有外源BMP4的原始hPSC中生成稳定,繁殖的TSC.
这些TSC模仿了第一季度的胎盘细胞,表现出内源性逆转录病毒基因激活,没有羊膜表达.
原始衍生的TSC可以维持超过30个通道,并分化为协同性细胞和异常性细胞.
在TS条件下诱导了TMSB4X,BMP5/7,GATA3和TFAP2A等特定基因.

结论:

化多能状态可以通过特定的信号通路调制直接产生TSC.
这种差异化路线绕过了天真的多能状态.
这些发现扩大了对hPSC分化潜力的理解,并提供了产生胎盘细胞的新方法.

更多相关视频

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Published on: July 25, 2016

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.