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带有形态素部分的亲光探针及其对选定的生物氧化剂的反应性

Julia Modrzejewska¹, Aleksandra Grzelakowska¹, Marcin Szala¹

¹Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 16, Lodz, Poland.

Luminescence : the journal of biological and chemical luminescence

|February 9, 2024

概括

一个新的光探测器,MpC-BA,有效地检测有害的生物氧化剂,如过氧和低酸. 该工具提供了改善的水溶性,稳定性和快速响应,用于在生物环境中检测这些氧化剂.

科学领域:

分析化学分析化学
生物化学生物化学
毒理学毒理学毒理学

背景情况:

生物氧化剂,包括过氧和低酸,在生理过程中起着关键作用,但它们的过度生产可能导致细胞损伤和疾病.
现有的检测这些氧化剂的方法有限,需要开发新的,敏感和特定的探针.
有酸组的库马林衍生物已经成为炎症氧化剂的有希望的光探针.

研究的目的:

为了合成和描述一种基于氨酸的新型光探针,4-[2-(morpholin-4-yl)-2-oxoethyl]-2-oxo-2H-chromen-7-yl酸 (MpC-BA).
评估探针的有效性,可溶性,稳定性和响应时间,用于检测生物氧化剂,特别是过氧和低酸.
研究MpC-BA与各种氧化剂相互作用时产生的反应通路和产品.

主要方法:

MpC-BA光探针的合成和特征.
光测量以评估探针对过氧酸盐 (ONOO−),低酸 (HOCl) 和过氧化 (H2O2) 的反应.
高性能液体染色学 (HPLC) 用于识别反应产物,包括化和减少.

主要成果:

MpC-BA表现出增强的水溶性,高稳定性,以及对过氧酸和低酸的快速反应.
过氧酸盐将MpC-BA氧化为MpC-OH,其中一个小途径产生了一种过氧酸盐特异性产品 (MpC-H).
低酸与MpC-BA反应形成MpC-OH,可以进一步化为特定的HOCl产物 (MpC-OHCl);过氧化的反应速度较慢.

结论:

MpC-BA是一种有前途的光探针,用于在生物环境中检测过氧酸和低酸.
探测器的独特反应产物有助于区分检测到的氧化剂.
使用HPLC的进一步验证补充了光数据,以准确识别氧化剂.

关键词:

基于库马林的探头.对氧化剂敏感的探头过氧化特异性产品,HOCl特异性产品,HPLC分析产品氧化还原探测器探测器

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