基于深度学习的量化和转录基因分析揭示了Cannabis sativa中甲基斯酸介导的腺状三组形成途径
Xiaoqin Huang1, Wei Chen1, Yuqing Zhao1
1Haixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
The Plant journal : for cell and molecular biology
|February 9, 2024
概括
研究人员开发了一个深度学习模型来跟踪大麻腺状三体的发展. 他们确定了一条涉及CsMYC4的甲基斯蒙酸途径,这对于大麻素生产至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物技术是生物技术.
- 基因组学就是基因组学.
背景情况:
- 大麻腺状三体 (GTs) 对于二次代谢物产生至关重要,但它们的发育调节知之甚少.
- 精确的量化和GT的发育概况对于了解其形成和优化大麻素产量至关重要.
研究的目的:
- 开发一个深度学习模型,以高效准确地检测和量化大麻GTs.
- 阐明大麻GT形成背后的分子机制和发育动态.
- 确定参与GT开发和二次代谢物生物合成的关键调节者.
主要方法:
- 开发大麻腺状三体检测模型 (CGTDM),这是一个深度学习算法.
- 基因表达在八个发育时间点中的概况.
- 与CGTDM测量相结合的权重基因共同表达网络分析.
- 外源性甲基斯酸盐 (MeJA) 治疗以研究GT的发展.
主要成果:
- CGTDM准确地区分和量化了三种类型的大麻GT.
- 在GT发育过程中捕获了基因表达,表型和代谢物的动态变化.
- 一个MeJA-响应的基因模块与跟踪GT密度和大麻素含量相关.
- 外源MeJA治疗促进了GT的发展,并确定了CsMYC4作为一个关键的积极调节者.
结论:
- 该研究为GT分析提供了新的计算工具,并对GT发展调节的洞察力.
- 由转录因子CsMYC4介导的甲基斯蒙酸信号传递,在大麻GT形成中发挥着关键作用.
- 这些发现支持了分子育种,基因工程和大麻中大麻素生产优化的进展.
相关概念视频
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02:04Regulation of Transpiration by Stomata
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...