一个全面的战略,通过指纹检测评估香粉的质量,并结合快速的定性和定量多成分分析
Zheyong Wang1, Yumeng Zhang1, Zixuan Fu1
1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
Phytochemical analysis : PCA
|February 9, 2024
概括
使用UPLC-FT-ICR-MS建立了香粉 (CAP) 质量控制,确定了58种化合物. 这种可靠的方法确保了对肠道疾病治疗和进一步研究的 CAP 质量的一致性.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 传统中国医药 传统中国医药
背景情况:
- 粉 (CAP) 是治疗肠道疾病的传统药物,但其材料基础需要进一步阐明.
- 有限的研究需要CAP的强有力的质量控制方法来确保有效性和安全性.
- 了解CAP的化学成分对于推进其功能机制研究至关重要.
研究的目的:
- 开发和验证长粉 (CAP) 的综合质量控制方法.
- 在质量和数量上分析CAP的化学成分.
- 建立指纹和内容确定方法,以评估农业农产品质量.
主要方法:
- 超高性能液态染色学与里埃变换离子循环子共振质谱学 (UPLC-FT-ICR-MS) 结合使用用于组件识别.
- 使用UPLC生成了10批CAP产品的化学指纹.
- 对关键组件进行了定量分析,以评估方法可靠性.
主要成果:
- 在CAP中,共有58种化合物被初步确定.
- 在10个CAP批次中观察到高度相似性 (>0.995),确定了23个共同峰值.
- 定量方法显示出优异的线性 (r2 ≥ 0.9992),精度 (<2% RSD) 和稳定性 (<2% RSD).
结论:
- 开发的UPLC-FT-ICR-MS方法有效地识别和量化了CAP的化学成分.
- 已建立的指纹和内容确定方法是稳定的,可靠的和用户友好的.
- 这种方法适用于常规质量控制,可以支持CAP的体内研究.
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