热挤出Morus alba叶对肠道微生物群和上皮细胞的作用
Hyun Bok Kim1, Eun Ji Go2, Jong-Suep Baek2,3,4
1National Institute of Agricultural Sciences, RDA, Wanju 55365, South Korea.
Heliyon
|February 9, 2024
概括
这项研究使用热挤出 (HME) 来开发了Morus alba叶子的持续释放配方. 该配方通过平衡肠道微生物和增强肠道上皮细胞来改善肠道健康.
科学领域:
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
- 材料科学 材料科学 材料科学
背景情况:
- 鲁丁和异基氨酸具有有益的作用,但由于溶解性差,阻碍了它们的应用.
- 白树的叶子是鲁丁和异基的天然来源.
- 开发稳定和可溶性配方对于治疗疗效至关重要.
研究的目的:
- 使用热挤出 (HME) 来创建 Morus alba 叶子的持续释放配方.
- 评估配方对肠道微生物平衡的影响.
- 评估配方对肠道上皮细胞健康和功能的影响.
主要方法:
- 使用 Morus alba 叶子和通过 HME.com 制备了一种持续释放的配方,并通过 HME.com 制备了一种水友性聚合物矩阵.
- 评估了对致病细菌 (大肠杆菌,金黄色葡萄球菌,菌菌) 的抗菌活性和益生菌的增殖 (拉克托巴西勒斯·拉姆诺斯,佩迪奥科克斯·托萨斯).
- 使用Caco-2和RAW 264.7细胞的共同培养模型来评估肠道上皮细胞的反应,包括在诱导脂多糖体 (LPS) 后的皮电阻和紧结蛋白表达.
主要成果:
- 经HME加工的Morus alba叶片配方显示出对病原体具有显著的抗菌活性.
- 该配方对有益益生菌的扩散产生了积极的影响.
- 在体外炎症模型中,该配方恢复了体电阻,增加了紧结蛋白水平,并降低了促炎性细胞因子表达.
结论:
- 热挤出 (HME) 的Morus alba叶产生一个稳定的,可溶性配方,改善生物可用性.
- 该配方有效调节肠道微生物群的组成,促进更健康的肠道环境.
- Morus alba叶HME配方支持肠道上皮质屏障的完整性,并减少炎症,表明有可能改善肠道健康.
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