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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Reporter Genes02:11

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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相关实验视频

Updated: Jul 4, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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评估Riboglow-FLIM探针用于RNA传感

Nadia Sarfraz1, Luke K Shafik1, Zachary R Stickelman1

  • 1Department of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.

RSC chemical biology
|February 9, 2024
PubMed
概括
此摘要是机器生成的。

瑞博格洛-FLIM通过测量光寿命,使得活细胞RNA的跟踪成为可能. 探针选择和细胞类型显著影响RNA检测,指导未来的工具开发.

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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 里博格洛-FLIM是一种用于在活细胞中基因标记和跟踪RNA分子的新技术.
  • 它依赖于测量与RNA标签结合的小分子探针的光寿命.
  • 这种方法为先进的RNA动态研究提供了潜力.

研究的目的:

  • 系统地和定量地评估Riboglow-FLIM技术的关键元素.
  • 评估探头设计 (链接器,光体) 和细胞环境对光寿命测量的影响.
  • 为Riboglow-FLIM应用程序和进一步工具开发奠定基础.

主要方法:

  • 在体外测量各种Riboglow-FLIM探针变体的光寿命.
  • 光终身成像显微镜 (FLIM) 在各种哺乳动物细胞类型中.
  • 代谢分析,以评估对细胞生理学的潜在影响.

主要成果:

  • 在探针变体中观察到明显的光寿命差异,这是由于链接器和光体变异.
  • 寿命变化取决于特定的探针和细胞类型.
  • 尽管观察到细胞系之间的寿命差异,但没有发现细胞健康的整体变化.

结论:

  • 探头选择和细胞环境是使用Riboglow-FLIM成功检测RNA的关键因素.
  • 该研究为优化Riboglow-FLIM用于各种生物系统提供了基础的见解.
  • 这项工作支持Riboglow-FLIM在各种研究领域的未来发展和应用.