鉴定具有诊断价值的性关联基因,用于性大动脉病的诊断
Chenxi Yu1, Yifeng Zhang1, Ling Yang2
1Department of Cardiovascular Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Frontiers in cardiovascular medicine
|February 9, 2024
概括
三个与火死相关的基因TREM1,TNFRSF11B和PGF被确定为动脉疾病 (CAVD) 的诊断生物标志物. 这些基因和相关的炎症微环境为CAVD提供了潜在的治疗点.
科学领域:
- 心血管研究研究心血管研究
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 动脉疾病 (CAVD) 是一种普遍的膜疾病,也是心脏手术的主要原因.
- 对于CAVD进展的潜在机制的理解仍然不完全.
- 研究与热病相关的基因为了解CAVD病原体提供了一种新的方法.
研究的目的:
- 为了确定与灭相关的基因,涉及到CAVD的发展.
- 建立使用与热致死相关的基因对CAVD的诊断模型.
- 探索已识别的生物标志物与CAVD中免疫细胞透之间的关系.
主要方法:
- 在公开的微阵列和RNA测序数据集上利用生物信息学分析 (GSE51472,GSE12644,GSE83453,GSE153555).
- 在正常和化大动脉之间识别出差异表达基因 (DEGs) 和与烧灭相关的DEGs.
- 采用LASSO回归和随机森林 (RF) 算法来选择诊断候选基因并构建诊断模型.
- 通过接受器操作特征 (ROC) 曲线分析验证诊断性能,并通过CIBERSORT分析评估免疫细胞透.
主要成果:
- 鉴定了805个DEG,其中17个与热相关的DEG被发现在CAVD中发生了显著的变化.
- 拉索和射频分析确定TREM1,TNFRSF11B和PGF作为CAVD的关键诊断候选基因.
- 在培训和验证队列中,诊断模型和个体基因表现出高诊断准确性 (AUC>0.75).
- 在M0巨细胞透和TREM1,TNFRSF11B和PGF的表达之间发现了正相关性.
结论:
- TREM1,TNFRSF11B和PGF被确定为CAVD的强大的诊断生物标志物.
- 这些与灭相关的基因代表了潜在的治疗点.
- 针对这些基因和亲炎性膜微环境可能为CAVD治疗提供新的策略.
更多相关视频
04:30Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
Published on: February 4, 2021
3.4K
05:47Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
Published on: May 10, 2021
4.0K
相关概念视频
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT
Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...
Mitral Stenosis II: Clinical features and Diagnostic Tests
Mitral stenosis is a heart condition in which the mitral valve, which allows blood to flow from the left atrium to the left ventricle, becomes narrowed or stenotic. This narrowing hinders blood flow and leads to clinical symptoms requiring specific medical evaluations and management strategies. The following overview outlines the clinical symptoms, assessments, diagnostic findings, prevention methods, and treatments for mitral stenosis.Clinical ManifestationsDyspnea (shortness of breath): This...
Aortic Regurgitation II: Clinical Features and Diagnostic Tests
Aortic valve regurgitation (AR) occurs when the aortic valve fails to close properly, allowing blood to flow backward from the aorta into the left ventricle. This backflow can result in two distinct clinical presentations: acute and chronic AR, each characterized by its own set of symptoms and physical findings.Acute Aortic RegurgitationAcute AR presents with a sudden onset of severe symptoms. Patients typically experience profound dyspnea (shortness of breath), chest pain, and signs of left...
