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暴露于吸入麻醉剂的兽医呈现染色体损伤,细胞亡和细胞周期变化

Mariana G Braz¹, Drielle B S Figueiredo¹, Marjorie A Golim²

¹Medical School, Sao Paulo State University - UNESP, Botucatu, Brazil.

Environmental and molecular mutagenesis

|February 9, 2024

概括

暴露于麻醉剂异氨酸的兽医显示DNA损伤增加和细胞周期改变. 这种职业暴露会影响淋巴细胞和血细胞的健康,突出显示兽医麻醉的潜在风险.

科学领域:

职业健康职业健康职业健康
毒理学毒理学毒理学
兽医医学兽医医学兽医医学

背景情况:

易挥发性麻醉剂如异氨酸在兽医中被广泛使用.
职业接触麻醉剂可能会给兽医专业人员带来健康风险.
关于异黄素暴露对兽医细胞健康的具体影响的研究有限.

研究的目的:

研究职业接触异黄素的兽医的淋巴细胞和血液细胞的DNA损伤,细胞活力和细胞周期变化.
量化尿液中异黄素水平作为暴露的生物标志物.
评估慢性异黄素暴露对兽医环境中细胞健康的影响.

主要方法:

截面研究比较了20名暴露于异黄素的兽医与20名未暴露的对照者.
在淋巴细胞中使用微核 (MN) 和玛-H2AX (γ-H2AX) 试验评估DNA损伤.
使用流式细胞计量评估细胞活力,细胞亡和细胞周期阶段.
通过头空间气色谱-质谱法量化尿液异二的量化.

主要成果:

与对照组相比,暴露的兽医患有显著更高的微核频率和更高的淋巴细胞亡率.
在暴露组中观察到G0/G1阶段的多态核细胞数量增加和G2/M阶段的比例减少.
在暴露组中,尿液中异黄素水平平均为25.7±23.7μg/L.
在职业暴露的兽医中,可活性淋巴细胞的统计显著减少.

结论:

对挥发性麻醉剂异氨酸的职业暴露与兽医的淋巴细胞中显著的染色体损伤和亡有关.
暴露于异氨酸会改变周围血液细胞的细胞循环分布,影响增殖.
这些发现强调了需要采取保护措施,以尽量减少兽医实践中的麻醉剂暴露.

关键词:

灭症 (apoptosis) 是一种死亡的过程.细胞循环的各个阶段.这是一个微核的微核.职业暴露职业暴露职业暴露挥发性麻醉剂是一种挥发性麻醉剂.

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