有效的一合成尿素二酸银糖,采用三酶系统
Yajing Li1, Qi Chen1, Siyao Liu1
1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Science, Jilin University, Changchun 130021, China.
Journal of agricultural and food chemistry
|February 9, 2024
概括
本研究提出了一种具有成本效益的,单一的方法来合成UDP-Gal (尿素二酸银),使用热友酶系统. 这一突破增强了对工业有价值的糖化物和益生菌的生产.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 碳水化合物化学 碳水化合物化学
背景情况:
- 核酸糖的高成本限制了糖化物和益生菌的工业应用.
- 对食品和制药行业来说,高效的UDP-Gal生物合成至关重要.
研究的目的:
- 为UDP-Gal生物合成开发一种具有成本效益的,单一的方法.
- 为了优化反应条件以获得高产量和时空产量 (STY).
主要方法:
- 使用热友多酶系统,包括GalK,UGPase和PPase.
- 采用料批和重复批反应策略.
- 研究了GalK的替代酸盐捐赠者.
主要成果:
- 在最佳条件下,在2小时内实现了87.4%的UTP转换.
- 累积33.76毫米的UDP-Gal,在料批反应中STY为6.36g/L·h-1.
- 经过证明的重复批次合成,在8个循环中平均产量为10.80g/L,生物催化剂负载低.
- 发现GalK具有使用ADP作为酸盐供体用于Gal-1P合成的新能力.
结论:
- 开发的单方法为UDP-Gal生产提供了高效率,低成本和系统稳定性.
- 提供了对快速UDP-Gal合成和高价值甘油酸化合物生产的新见解.
- 加尔克对ADP的新用途扩大了酶的可能性.
相关概念视频
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