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Updated: Jul 19, 2026

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
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Published on: January 28, 2015

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用于通过神经原蛋白-2介导诱导人体干细胞衍生的神经原生细胞的协议

Ellen J Guss1, Laila Sathe2, Alexander Dai2

  • 1Department of Stem Cell and Regenerative Biology, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.

STAR protocols
|February 9, 2024
PubMed
概括

这项研究引入了一种快速,可靠的方法,从人类干细胞中创建神经前代细胞 (NPC). 这种技术有助于研究大脑发育和自闭症等疾病.

关键词:
细胞培养培养的细胞培养.神经科学是一个神经科学.干细胞是干细胞.

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
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Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.