对于TCRαβ+CD8αα+内皮细胞淋巴细胞系的胸膜发育,BCL6是必要的
Qi Xing1,2, Dehui Chang2, Shiyuan Xie3
1Shanghai Immune Therapy Institute, New Cornerstone Science Laboratory, Shanghai Jiao Tong University School of Medicine-affiliated Renji Hospital, Shanghai 200127, China.
Science immunology
|February 9, 2024
概括
转录因子BCL6对于专门的肠道T细胞的发展至关重要,称为TCRαβ+CD8αα+内皮淋巴细胞 (CD8αα+αβ IELs). 甲状腺中甲状腺缺乏会损害这些关键免疫细胞的生成和成熟.
科学领域:
- 免疫学 免疫学 免疫学
- T细胞生物学T细胞生物学
- 免疫细胞发育的分子机制
背景情况:
- TCRαβ+CD8αα+内皮淋巴细胞 (CD8αα+αβ IELs) 是位于肠表皮中的一个专门的T细胞子集.
- 这些细胞来源于通过主激素依赖途径选择的胸膜IEL前体 (IELps).
- 控制CD8αα+αβIELs胸膜选择和分化的精确分子途径在很大程度上仍未被阐明.
研究的目的:
- 研究转录因子BCL6在CD8αα+αβIELs胸膜发育中的作用.
- 确定BCL6影响IELp生成和分化的分子机制.
主要方法:
- 在野生型和BCL6缺乏的小鼠中分析T细胞.
- 流细胞测量用于识别和量化特定的T细胞种群 (例如CD8αα+αβIELs,PD1+IELps).
- 对胸细胞亡和IELp分化标志物的评估.
主要成果:
- 在αβ T细胞中Bcl6缺乏导致CD8αα+αβ IELs几乎完全缺失.
- 在相当一部分成熟的CD8αα+αβIEL和胸膜PD1+IEL中检测到BCL6表达.
- Bcl6 缺乏导致早期 IELp 生成受损,在胸膜选择过程中增加了 IELp 前体的亡,并阻碍了 IELp 的分化.
结论:
- BCL6是 CD8αα+αβ IELs 胸膜发育不可或缺的关键转录因子.
- 胸膜TCR信号诱导IELps中的BCL6表达以ERK依赖的方式.
- BCL6调节IELp前体的生存和分化,确保适当地产生肠内 CD8αα+αβ IELs.
更多相关视频
08:56Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
Published on: June 9, 2015
8.3K
09:41Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
Published on: July 26, 2024
760
相关概念视频
01:22T Cell Activation and Clonal Selection
733
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
733
01:23Cells of the Adaptive Immune Response
987
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
987
01:24B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
01:24T Cell Types and Functions
1.0K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.0K
01:28Diversity of Antigen Receptors
589
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
589
01:20Special Features of Adaptive Immunity
820
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
820