相关实验视频

Updated: Jul 4, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

用添加的碳点促进CRISPR/Cas减少环境中的抗生素耐药性基因

Feiran Chen^1,2, Hao Du^1,2, Mengna Tao^1,2

¹Institute of Environmental Processes and Pollution Control, and School of Environment and Ecology, Jiangnan University, Wuxi 214122, China.

Journal of agricultural and food chemistry

|February 9, 2024

概括

用添加的碳点传递CRISPR-Cas9以消除细菌中的高风险抗生素耐药性基因 (ARG). 这种方法使土壤中的耐药细菌重新敏感,提供了一种减少环境ARG储存的策略.

科学领域:

环境微生物学环境微生物学
分子生物学分子生物学
纳米技术纳米技术

背景情况:

抗生素耐药性基因 (ARG) 构成了全球健康威胁.
ARG在环境中的传播使抵抗管理复杂化.
在现场针对ARG对于控制传播至关重要.

研究的目的:

开发一种精确的方法来准和消除环境中的多药ARG.
为了有效地传递CRISPR-Cas9/sgRNAs,利用添加的碳点 (NCDs).
在复杂的环境矩阵中重新敏感化抗生素耐药细菌.

主要方法:

开发了针对高风险ARG (tet,cat,aph(3 extquotesingle) -Ia) 的CRISPR-Cas9/sgRNAs,这些RNAs可以被使用.
用添加的碳点 (NCD) 被用作Cas9/sgRNAs的输送载体.
进行了NCD与Cas9蛋白比率的优化.
通过NCD传递的CRISPR-Cas9的疗效在大肠杆菌和土壤环境中进行了测试.

主要成果:

非传染性疾病促进了Cas9/sgRNAs向大肠杆菌的非细胞毒性传递,消除了目标ARGs.
优化的NCD实现了持续的ARG消除.
非传染性疾病使Cas9/多个sgRNAs能够使土壤中50%的抗生素耐药细菌重新敏感.
在复杂的土壤环境中,Cas9/多个sgRNA单独是无效的.

结论:

非传染性疾病为CRISPR-Cas9/sgRNAs提供了一种有效且非细胞毒的输送系统,用于对抗ARG.
该战略显示了最大限度地减少环境ARG水库的巨大潜力,特别是在农业系统中.
该方法提供了一种精确而快速的方法,用于管理复杂环境中的抗生素耐药性.

关键词:

克里斯普尔-Cas9是什么意思抗生素耐药性基因是抗生素耐药性的基因.在土壤中多重裂变.纳米配送是一种纳米配送.用剂添加碳点的碳点.

更多相关视频

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Antibiotic Selection

Antibiotic Selection

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...