通过YAP/TAZ信号传递,M2巨细胞通过YAP/TAZ信号传递促进子结膜纤维化
Yiwei Wang1, Xingchen Geng1, Zhihua Guo1
1Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, China.
Annals of medicine
|February 9, 2024
概括
通过TGF-β1/Smad2/3-YAP/TAZ通路激活人类的纤维细胞,M2巨在植入后促进了亚结合膜纤维化. 向M2巨细胞提供了一个潜在的抗纤维性治疗策略.
科学领域:
- 眼科医生 眼科 眼科
- 免疫学 免疫学 免疫学
- 纤维化研究 纤维化研究
背景情况:
- 副结膜纤维化是眼科手术后的严重并发症,包括植入物.
- 了解驱动纤维化细胞和分子机制对于开发有效治疗方法至关重要.
- M2巨细胞与组织重塑和纤维化有关,但它们在植入后纤维化中的特定作用需要阐明.
研究的目的:
- 研究M2巨细胞在植入物 (SI) 诱导的副结膜纤维化中的作用.
- 探索M2巨细胞对纤维化有所贡献的潜在分子机制.
- 评估针对M2巨细胞进行抗纤维化策略的治疗潜力.
主要方法:
- 使用SI手术建立了子子结膜纤维化模型.
- 组织病理学被用来评估M2巨细胞的分布和原沉积.
- 试验室研究评估了M2细胞对人类子纤维细胞 (HTFs) 迁移和激活的作用,使用伤口划伤试验,免疫光和西部涂抹.
主要成果:
- 在手术后2周,在植入物周围观察到M2巨细胞 (CD68+/CD206+细胞) 的透增加,与8周密集的原沉积相关.
- M2巨细胞表达了高水平的CD206和转化生长因子-β1 (TGF-β1).
- 通过对TGF-β1/Smad2/3-YAP/TAZ通路进行上调,M2条件的介质增强了HTF迁移和细胞外基质合成 (原I,纤维内素). 一种YAP抑制剂Verteporfin阻断了这些效应.
结论:
- 在植入后促进子结膜纤维化方面,M2巨细胞发挥着关键作用.
- TGF-β1/Smad2/3-YAP/TAZ信号通路参与HTF中M2巨细胞介导的纤维化活动.
- 在眼部条件下,M2巨体是抗纤维性干预的有希望的治疗点.
相关概念视频
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K


